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10.1186/s12934-016-0508-5 http://scihub22266oqcxt.onion/10.1186/s12934-016-0508-5 C4902970!4902970 !27287473     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27287473 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Microb+Cell+Fact 2016 ; 15 (1 ): 104 Nephropedia Template TP {{tp|p=27287473 |t=2016. Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei |pdf=|usr=}}{{27287473 }} gab.com Text Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei JO: Microb Cell Fact http://www.kidney.de/pget.php?&tw=1&pmid=27287473 #FOAvip BACKGROUND: The filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins at low cost. We have developed T. reesei strains suitable for production of therapeutic proteins by reducing the secreted protease activity. Protease activity has been the major hindrance to achieving high production levels. We have constructed a series of interferon alpha-2b (IFN?-2b) production strains with 9 protease deletions to gain knowledge for further strain development. RESULTS: We have identified two protease deletions that dramatically improved the production levels. Deletion of the subtilisin protease slp7 and the metalloprotease amp2 has enabled production levels of IFN?-2b up to 2.1 and 2.4 g/L, respectively. With addition of soybean trypsin protease inhibitor the level of production improved to 4.5 g/L, with an additional 1.8 g/L still bound to the secretion carrier protein. CONCLUSIONS: High levels of IFN?-2b were produced using T. reesei strains with reduced protease secretion. Further strain development can be done to improve the production system by reducing protease activity and improving carrier protein cleavage. Twit Text FOAVip Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei ????Microb Cell Fact http://www.kidney.de/pget.php?&tw=1&pmid=27287473 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27287473 #FOAvip English Wikipedia <ref>{{Cite pmid|27287473 }}</ref> Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei #MMPMID27287473 Landowski CP ; Mustalahti E ; Wahl R ; Croute L ; Sivasiddarthan D ; Westerholm-Parvinen A ; Sommer B ; Ostermeier C ; Helk B ; Saarinen J ; Saloheimo M Microb Cell Fact 2016[Jun]; 15 (1 ): 104 PMID27287473 show ga BACKGROUND: The filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an excellent host system for production of high levels of therapeutic proteins at low cost. We have developed T. reesei strains suitable for production of therapeutic proteins by reducing the secreted protease activity. Protease activity has been the major hindrance to achieving high production levels. We have constructed a series of interferon alpha-2b (IFN?-2b) production strains with 9 protease deletions to gain knowledge for further strain development. RESULTS: We have identified two protease deletions that dramatically improved the production levels. Deletion of the subtilisin protease slp7 and the metalloprotease amp2 has enabled production levels of IFN?-2b up to 2.1 and 2.4 g/L, respectively. With addition of soybean trypsin protease inhibitor the level of production improved to 4.5 g/L, with an additional 1.8 g/L still bound to the secretion carrier protein. CONCLUSIONS: High levels of IFN?-2b were produced using T. reesei strains with reduced protease secretion. Further strain development can be done to improve the production system by reducing protease activity and improving carrier protein cleavage. |Bioreactors [MESH] |Interferon alpha-2 [MESH] |Interferon-alpha/*biosynthesis/economics/genetics [MESH] |Peptide Hydrolases/genetics/metabolism [MESH] |Recombinant Proteins/*biosynthesis/economics/genetics [MESH] |Trichoderma/genetics/growth & development/*metabolism [MESH]Enabling low cost biopharmaceuticals: high level interferon alpha-2b p(epmc).pdf DeepDyve Pubget Overpricing