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10.1038/srep27722 http://scihub22266oqcxt.onion/10.1038/srep27722 C4899811!4899811 !27278669     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27278669 &cmd=llinks): Failed to open stream: HTTP request failed! 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Genomic leftovers: identifying novel microsatellites, over-represented motifs and functional elements in the human genome |pdf=|usr=}}{{27278669 }} gab.com Text Genomic leftovers: identifying novel microsatellites, over-represented motifs and functional elements in the human genome JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27278669 #FOAvip The human genome is 99% complete. This study contributes to filling the 1% gap by enriching previously unknown repeat regions called microsatellites (MST). We devised a Global MST Enrichment (GME) kit to enrich and nextgen sequence 2 colorectal cell lines and 16 normal human samples to illustrate its utility in identifying contigs from reads that do not map to the genome reference. The analysis of these samples yielded 790 novel extra-referential concordant contigs that are observed in more than one sample. We searched for evidence of functional elements in the concordant contigs in two ways: (1) BLAST-ing each contig against normal RNA-Seq samples, (2) Checking for predicted functional elements using GlimmerHMM. Of the 790 concordant contigs, 37 had an exact match to at least one RNA-Seq read; 15 aligned to more than 100 RNA-Seq reads. Of the 249 concordant contigs predicted by GlimmerHMM to have functional elements, 6 had at least one exact RNA-Seq match. BLAST-ing these novel contigs against all publically available sequences confirmed that they were found in human and chimpanzee BAC and FOSMID clones sequenced as part of the original human genome project. These extra-referential contigs predominantly contained pentameric repeats, especially two motifs: AATGG and GTGGA. Twit Text FOAVip Genomic leftovers: identifying novel microsatellites, over-represented motifs and functional elements in the human genome ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27278669 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27278669 #FOAvip English Wikipedia <ref>{{Cite pmid|27278669 }}</ref> Genomic leftovers: identifying novel microsatellites, over-represented motifs and functional elements in the human genome #MMPMID27278669 Fonville NC ; Velmurugan KR ; Tae H ; Vaksman Z ; McIver LJ ; Garner HR Sci Rep 2016[Jun]; 6 (ä): 27722 PMID27278669 show ga The human genome is 99% complete. This study contributes to filling the 1% gap by enriching previously unknown repeat regions called microsatellites (MST). We devised a Global MST Enrichment (GME) kit to enrich and nextgen sequence 2 colorectal cell lines and 16 normal human samples to illustrate its utility in identifying contigs from reads that do not map to the genome reference. The analysis of these samples yielded 790 novel extra-referential concordant contigs that are observed in more than one sample. We searched for evidence of functional elements in the concordant contigs in two ways: (1) BLAST-ing each contig against normal RNA-Seq samples, (2) Checking for predicted functional elements using GlimmerHMM. Of the 790 concordant contigs, 37 had an exact match to at least one RNA-Seq read; 15 aligned to more than 100 RNA-Seq reads. Of the 249 concordant contigs predicted by GlimmerHMM to have functional elements, 6 had at least one exact RNA-Seq match. BLAST-ing these novel contigs against all publically available sequences confirmed that they were found in human and chimpanzee BAC and FOSMID clones sequenced as part of the original human genome project. These extra-referential contigs predominantly contained pentameric repeats, especially two motifs: AATGG and GTGGA. |*Microsatellite Repeats [MESH] |Algorithms [MESH] |Animals [MESH] |Cell Line [MESH] |Contig Mapping [MESH] |Genome, Human [MESH] |Genomics [MESH] |Humans [MESH] |Pan troglodytes/genetics [MESH] |Sequence Analysis, DNA/*methods [MESH]Genomic leftovers: identifying novel microsatellites, over-represented(epmc).pdf DeepDyve Pubget Overpricing