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10.1016/j.bone.2016.03.018 http://scihub22266oqcxt.onion/10.1016/j.bone.2016.03.018 C4899086!4899086 !27080510     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27080510 &cmd=llinks): Failed to open stream: HTTP request failed! 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Selective enrichment of microRNAs in extracellular matrix vesicles produced by growth plate chondrocytes |pdf=|usr=}}{{27080510 }} gab.com Text Selective enrichment of microRNAs in extracellular matrix vesicles produced by growth plate chondrocytes JO: Bone http://www.kidney.de/pget.php?&tw=1&pmid=27080510 #FOAvip Matrix vesicles (MVs) are membrane organelles found in the extracellular matrix of calcifying cells, which contain matrix processing enzymes and regulate the extracellular environment via action of these enzymes. It is unknown whether MVs are also exosomic mediators of cell-cell communication via transfer of RNA material, and specifically, microRNA (miRNA). We investigated the presence of RNA in MVs isolated from cultures of costochondral growth zone chondrocytes. Our results showed that the average yield of MV RNA was 1.93±0.78ng RNA/10(4) cells, which was approximately 0.1% of the parent cell's total RNA. MV RNA was well-protected from RNase by the lipid membrane and was highly enriched in small RNA molecules compared to cells. Moreover, coding and non-coding small RNAs in MVs were in proportions that differed from parent cells. Enrichment of specific miRNAs was consistently observed in all three miRNA detection platforms that we used, suggesting that miRNAs are selectively packaged into MVs. MV-enriched miRNAs were related to different signaling pathways associated with bone formation. This study suggests a significant role for MVs as "matrisomes" in cell-cell communication in cartilage and bone development via transfer of specific miRNAs. Twit Text FOAVip Selective enrichment of microRNAs in extracellular matrix vesicles produced by growth plate chondrocytes ????Bone http://www.kidney.de/pget.php?&tw=1&pmid=27080510 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27080510 #FOAvip English Wikipedia <ref>{{Cite pmid|27080510 }}</ref> Selective enrichment of microRNAs in extracellular matrix vesicles produced by growth plate chondrocytes #MMPMID27080510 Lin Z ; Rodriguez NE ; Zhao J ; Ramey AN ; Hyzy SL ; Boyan BD ; Schwartz Z Bone 2016[Jul]; 88 (ä): 47-55 PMID27080510 show ga Matrix vesicles (MVs) are membrane organelles found in the extracellular matrix of calcifying cells, which contain matrix processing enzymes and regulate the extracellular environment via action of these enzymes. It is unknown whether MVs are also exosomic mediators of cell-cell communication via transfer of RNA material, and specifically, microRNA (miRNA). We investigated the presence of RNA in MVs isolated from cultures of costochondral growth zone chondrocytes. Our results showed that the average yield of MV RNA was 1.93±0.78ng RNA/10(4) cells, which was approximately 0.1% of the parent cell's total RNA. MV RNA was well-protected from RNase by the lipid membrane and was highly enriched in small RNA molecules compared to cells. Moreover, coding and non-coding small RNAs in MVs were in proportions that differed from parent cells. Enrichment of specific miRNAs was consistently observed in all three miRNA detection platforms that we used, suggesting that miRNAs are selectively packaged into MVs. MV-enriched miRNAs were related to different signaling pathways associated with bone formation. This study suggests a significant role for MVs as "matrisomes" in cell-cell communication in cartilage and bone development via transfer of specific miRNAs. |Animals [MESH] |Chondrocytes/*metabolism [MESH] |Extracellular Matrix/*metabolism [MESH] |Extracellular Vesicles/*metabolism [MESH] |Growth Plate/*metabolism [MESH] |Male [MESH] |MicroRNAs/*metabolism [MESH] |Molecular Sequence Annotation [MESH] |Rats, Sprague-Dawley [MESH] |Reproducibility of Results [MESH]Selective enrichment of microRNAs in extracellular matrix vesicles pro(epmc).pdf DeepDyve Pubget Overpricing