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Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian
skin to enclose the embryonic body
#MMPMID26989131
Panousopoulou E
; Hobbs C
; Mason I
; Green JB
; Formstone CJ
J Cell Sci
2016[May]; 129
(9
): 1915-27
PMID26989131
show ga
Epiboly is a morphogenetic process that is employed in the surface ectoderm of
anamniotes during gastrulation to cover the entire embryo. We propose here that
mammals also utilise this process to expand the epidermis and enclose the body
cavity and spinal cord with a protective surface covering. Our data supports a
model whereby epidermal spreading is driven by the primary establishment of the
epidermal basal progenitor monolayer through radial cell intercalation of a
multi-layered epithelium towards the basal lamina. By using a suspension
organotypic culture strategy, we find that this process is fibronectin-dependent
and autonomous to the skin. The radial cell rearrangements that drive epidermal
spreading also require ROCK activity but are driven by cell protrusions and not
myosin II contractility. Epidermal progenitor monolayer formation and epidermal
spreading are delayed in Crash mice, which possess a dominant mutation in Celsr1,
an orthologue of the core planar cell polarity (PCP) Drosophila protein Flamingo
(also known as Stan). We observe a failure of ventral enclosure in Crash mutants
suggesting that defective epidermal spreading might underlie some ventral wall
birth defects.
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