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10.1038/srep27290 http://scihub22266oqcxt.onion/10.1038/srep27290 C4893670!4893670 !27264341     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27264341 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2016 ; 6 (ä): 27290 Nephropedia Template TP {{tp|p=27264341 |t=2016. Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison |pdf=|usr=}}{{27264341 }} gab.com Text Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27264341 #FOAvip Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution beyond the classical diffraction-limit. Here, we compare the individual strengths and weaknesses of each technique by imaging a variety of different subcellular structures in fixed cells. We chose examples ranging from well separated vesicles to densely packed three dimensional filaments. We used quantitative and correlative analyses to assess the performance of SIM, STED and SMLM with the aim of establishing a rough guideline regarding the suitability for typical applications and to highlight pitfalls associated with the different techniques. Twit Text FOAVip Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27264341 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27264341 #FOAvip English Wikipedia <ref>{{Cite pmid|27264341 }}</ref> Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison #MMPMID27264341 Wegel E ; Göhler A ; Lagerholm BC ; Wainman A ; Uphoff S ; Kaufmann R ; Dobbie IM Sci Rep 2016[Jun]; 6 (ä): 27290 PMID27264341 show ga Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution beyond the classical diffraction-limit. Here, we compare the individual strengths and weaknesses of each technique by imaging a variety of different subcellular structures in fixed cells. We chose examples ranging from well separated vesicles to densely packed three dimensional filaments. We used quantitative and correlative analyses to assess the performance of SIM, STED and SMLM with the aim of establishing a rough guideline regarding the suitability for typical applications and to highlight pitfalls associated with the different techniques. |Animals [MESH] |COS Cells [MESH] |Chlorocebus aethiops [MESH] |Humans [MESH] |Macrophages/*cytology [MESH] |Microscopy, Fluorescence [MESH] |Microtubules/*ultrastructure [MESH] |Optical Imaging/*methods [MESH]Imaging cellular structures in super-resolution with SIM, STED and Loc(epmc).pdf DeepDyve Pubget Overpricing