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2013 ; 2013
(ä): 180439
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Comparison of the Kato-Katz, Wet Mount, and Formol-Ether Concentration Diagnostic
Techniques for Intestinal Helminth Infections in Ethiopia
#MMPMID27335845
Endris M
; Tekeste Z
; Lemma W
; Kassu A
ISRN Parasitol
2013[]; 2013
(ä): 180439
PMID27335845
show ga
Objective. The aim of this study was to evaluate the operational characteristics
(sensitivity and negative predictive value (NPV)) of wet mount, formol-ether
concentration (FEC), and Kato-Katz techniques for the determination of intestinal
parasitic infections. Method. A total of 354 faecal specimens were collected from
students in Northwest Ethiopia and screened with Kato-Katz, wet mount, and FEC
for the presence of intestinal parasitic infection. Since a gold standard test is
not available for detection of intestinal parasites, the combined results from
the three methods were used as diagnostic gold standard. Result. The prevalences
of intestinal parasites using the single wet mount, FEC, and Kato-Katz thick
smear techniques were 38.4%, 57.1%, and 59%, respectively. Taking the combined
results of three techniques as a standard test for intestinal parasitic
infection, the sensitivity and negative predictive value of Kato-Katz is 81.0%
(confidence interval (CI)?=?0.793-0.810) and 66.2% (CI?=?0.63-0.622),
respectively. The FEC detected 56 negative samples that were positive by the gold
standard, indicating 78.3% (CI?=?0.766-0.783) and 63.2% (CI?=?0.603-63)
sensitivity and NPV, respectively. Furthermore, Kato-Katz detects 113 cases that
were negative by a single wet mount. The ? agreement between the wet mount and
Kato-Katz methods for the diagnosis of Ascaris lumbricoides and hookworm was
substantial (? = 0.61 for Ascaris lumbricoides, ? = 0.65 for hookworm).