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10.1038/srep27179 http://scihub22266oqcxt.onion/10.1038/srep27179 C4890032!4890032!27251472     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP {{tp|p=27251472|t=2016. Dissecting the role of transforming growth factor-?1 in topmouth culter immunobiological activity: a fundamental functional analysis |pdf=|usr=}}{{27251472}} gab.com Text Dissecting the role of transforming growth factor- 1 in topmouth culter immunobiological activity: a fundamental functional analysis JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27251472 #FOAvip Transforming growth factor-?1 (TGF-?1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-?1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-?1 molecule in the topmouth culter. TGF-?1 is highly expressed in immune-related tissues of the culter, including the thymus, head kidney, and spleen. The recombinant culter TGF-?1 (cTGF-?1) was successfully expressed and purified in vitro, and the effects of cTGF-?1 on the mRNA expression of pro-inflammatory cytokines, such as TNF-? and IL-1?, in the absence or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-?1 was found to have bipolar properties in inflammatory reactions. Additionally, to assess the immune role of teleost TGF-?1 in vivo, the expression of TGF-?1 in the culter thymus and spleen tissues induced by poly I:C were also examined. The expression of TGF-?1 was obviously up-regulated, as shown in the cell lines. However, the peak time of cTGF-?1 expression in the cell lines occurred significantly earlier than in the organic tissues under the same inducer, suggesting that the response of the teleost TGF-?1 molecule to exogenous infection depends on a more complicated signalling pathway in vivo than in vitro. Twit Text FOAVip Dissecting the role of transforming growth factor- 1 in topmouth culter immunobiological activity: a fundamental functional analysis ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27251472 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27251472 #FOAvip English Wikipedia <ref>{{Cite pmid|27251472}}</ref> Dissecting the role of transforming growth factor-?1 in topmouth culter immunobiological activity: a fundamental functional analysis #MMPMID27251472 Qi P; Xie C; Guo B; Wu C Sci Rep 2016[]; 6 (ä): ä PMID27251472show ga Transforming growth factor-?1 (TGF-?1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-?1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-?1 molecule in the topmouth culter. TGF-?1 is highly expressed in immune-related tissues of the culter, including the thymus, head kidney, and spleen. The recombinant culter TGF-?1 (cTGF-?1) was successfully expressed and purified in vitro, and the effects of cTGF-?1 on the mRNA expression of pro-inflammatory cytokines, such as TNF-? and IL-1?, in the absence or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-?1 was found to have bipolar properties in inflammatory reactions. Additionally, to assess the immune role of teleost TGF-?1 in vivo, the expression of TGF-?1 in the culter thymus and spleen tissues induced by poly I:C were also examined. The expression of TGF-?1 was obviously up-regulated, as shown in the cell lines. However, the peak time of cTGF-?1 expression in the cell lines occurred significantly earlier than in the organic tissues under the same inducer, suggesting that the response of the teleost TGF-?1 molecule to exogenous infection depends on a more complicated signalling pathway in vivo than in vitro. äDissecting the role of transforming growth factor-?1 in topmouth culte(epmc).pdf DeepDyve Pubget Overpricing