Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27251472
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Dissecting the role of transforming growth factor-?1 in topmouth culter
immunobiological activity: a fundamental functional analysis
#MMPMID27251472
Qi P
; Xie C
; Guo B
; Wu C
Sci Rep
2016[Jun]; 6
(?): 27179
PMID27251472
show ga
Transforming growth factor-?1 (TGF-?1) has been proven to function primarily in
mammalian immunobiological activity, but information regarding the immune role of
TGF-?1 in teleosts is limited. In the present study, we describe the cDNA cloning
and characterization of the TGF-?1 molecule in the topmouth culter. TGF-?1 is
highly expressed in immune-related tissues of the culter, including the thymus,
head kidney, and spleen. The recombinant culter TGF-?1 (cTGF-?1) was successfully
expressed and purified in vitro, and the effects of cTGF-?1 on the mRNA
expression of pro-inflammatory cytokines, such as TNF-? and IL-1?, in the absence
or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-?1
was found to have bipolar properties in inflammatory reactions. Additionally, to
assess the immune role of teleost TGF-?1 in vivo, the expression of TGF-?1 in the
culter thymus and spleen tissues induced by poly I:C were also examined. The
expression of TGF-?1 was obviously up-regulated, as shown in the cell lines.
However, the peak time of cTGF-?1 expression in the cell lines occurred
significantly earlier than in the organic tissues under the same inducer,
suggesting that the response of the teleost TGF-?1 molecule to exogenous
infection depends on a more complicated signalling pathway in vivo than in vitro.
free
free
free
