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10.1038/nmeth.3810 http://scihub22266oqcxt.onion/10.1038/nmeth.3810 C4887338!4887338!27018577     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27018577.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2016 ; 13 (6): 508-14 Nephropedia Template TP {{tp|p=27018577|t=2016. Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP) |pdf=|usr=}}{{27018577}} gab.com Text Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP) JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=27018577 #FOAvip As RNA binding proteins (RBPs) play essential roles in cellular physiology by interacting with target RNAs, binding site identification by UV-crosslinking and immunoprecipitation (CLIP) of ribonucleoprotein complexes is critical to understanding RBP function. However, current CLIP protocols are technically demanding and yield low complexity libraries with high experimental failure rates. We have developed an enhanced CLIP (eCLIP) protocol that decreases requisite amplification by ~1,000-fold, decreasing discarded PCR duplicate reads by ~60% while maintaining single-nucleotide binding resolution. By simplifying the generation of paired IgG and size-matched input controls, eCLIP improves specificity in discovery of authentic binding sites. We generated 102 eCLIP experiments for 73 diverse RBPs in HepG2 and K562 cells (available at https://www.encodeproject.org), demonstrating that eCLIP enables large-scale and robust profiling, with amplification and sample requirements similar to ChIP-seq. eCLIP enables integrative analysis of diverse RBPs to reveal factor-specific profiles, common artifacts for CLIP and RNA-centric perspectives of RBP activity. Twit Text FOAVip Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP) ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=27018577 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27018577 #FOAvip English Wikipedia <ref>{{Cite pmid|27018577}}</ref> Robust transcriptome-wide discovery of RNA binding protein binding sites with enhanced CLIP (eCLIP) #MMPMID27018577 Van Nostrand EL; Pratt GA; Shishkin AA; Gelboin-Burkhart C; Fang MY; Sundararaman B; Blue SM; Nguyen TB; Surka C; Elkins K; Stanton R; Rigo F; Guttman M; Yeo GW Nat Methods 2016[Jun]; 13 (6): 508-14 PMID27018577show ga As RNA binding proteins (RBPs) play essential roles in cellular physiology by interacting with target RNAs, binding site identification by UV-crosslinking and immunoprecipitation (CLIP) of ribonucleoprotein complexes is critical to understanding RBP function. However, current CLIP protocols are technically demanding and yield low complexity libraries with high experimental failure rates. We have developed an enhanced CLIP (eCLIP) protocol that decreases requisite amplification by ~1,000-fold, decreasing discarded PCR duplicate reads by ~60% while maintaining single-nucleotide binding resolution. By simplifying the generation of paired IgG and size-matched input controls, eCLIP improves specificity in discovery of authentic binding sites. We generated 102 eCLIP experiments for 73 diverse RBPs in HepG2 and K562 cells (available at https://www.encodeproject.org), demonstrating that eCLIP enables large-scale and robust profiling, with amplification and sample requirements similar to ChIP-seq. eCLIP enables integrative analysis of diverse RBPs to reveal factor-specific profiles, common artifacts for CLIP and RNA-centric perspectives of RBP activity. äRobust transcriptome-wide discovery of RNA binding protein binding sit(epmc).pdf DeepDyve Pubget Overpricing