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10.1002/jcb.25226 http://scihub22266oqcxt.onion/10.1002/jcb.25226 C4886866!4886866 !25989233     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25989233 &cmd=llinks): Failed to open stream: HTTP request failed! 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Artificial zinc finger DNA binding domains: versatile tools for genome engineering and modulation of gene expression |pdf=|usr=}}{{25989233 }} gab.com Text Artificial zinc finger DNA binding domains: versatile tools for genome engineering and modulation of gene expression JO: J Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=25989233 #FOAvip Genome editing and alteration of gene expression by synthetic DNA binding activities gained a lot of momentum over the last decade. This is due to the development of new DNA binding molecules with enhanced binding specificity. The most commonly used DNA binding modules are zinc fingers (ZFs), TALE-domains, and the RNA component of the CRISPR/Cas9 system. These binding modules are fused or linked to either nucleases that cut the DNA and induce DNA repair processes, or to protein domains that activate or repress transcription of genes close to the targeted site in the genome. This review focuses on the structure, design, and applications of ZF DNA binding domains (ZFDBDs). ZFDBDs are relatively small and have been shown to penetrate the cell membrane without additional tags suggesting that they could be delivered to cells without a DNA or RNA intermediate. Advanced algorithms that are based on extensive knowledge of the mode of ZF/DNA interactions are used to design the amino acid composition of ZFDBDs so that they bind to unique sites in the genome. Off-target binding has been a concern for all synthetic DNA binding molecules. Thus, increasing the specificity and affinity of ZFDBDs will have a significant impact on their use in analytical or therapeutic settings. Twit Text FOAVip Artificial zinc finger DNA binding domains: versatile tools for genome engineering and modulation of gene expression ????J Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=25989233 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25989233 #FOAvip English Wikipedia <ref>{{Cite pmid|25989233 }}</ref> Artificial zinc finger DNA binding domains: versatile tools for genome engineering and modulation of gene expression #MMPMID25989233 Hossain MA ; Barrow JJ ; Shen Y ; Haq MI ; Bungert J J Cell Biochem 2015[Nov]; 116 (11 ): 2435-44 PMID25989233 show ga Genome editing and alteration of gene expression by synthetic DNA binding activities gained a lot of momentum over the last decade. This is due to the development of new DNA binding molecules with enhanced binding specificity. The most commonly used DNA binding modules are zinc fingers (ZFs), TALE-domains, and the RNA component of the CRISPR/Cas9 system. These binding modules are fused or linked to either nucleases that cut the DNA and induce DNA repair processes, or to protein domains that activate or repress transcription of genes close to the targeted site in the genome. This review focuses on the structure, design, and applications of ZF DNA binding domains (ZFDBDs). ZFDBDs are relatively small and have been shown to penetrate the cell membrane without additional tags suggesting that they could be delivered to cells without a DNA or RNA intermediate. Advanced algorithms that are based on extensive knowledge of the mode of ZF/DNA interactions are used to design the amino acid composition of ZFDBDs so that they bind to unique sites in the genome. Off-target binding has been a concern for all synthetic DNA binding molecules. Thus, increasing the specificity and affinity of ZFDBDs will have a significant impact on their use in analytical or therapeutic settings. |*Zinc Fingers [MESH] |Algorithms [MESH] |Binding Sites [MESH] |DNA-Binding Proteins/*chemistry/metabolism [MESH] |DNA/*metabolism [MESH] |Gene Expression Regulation [MESH] |Models, Molecular [MESH] |Protein Binding [MESH]Artificial zinc finger DNA binding domains: versatile tools for genome(epmc).pdf DeepDyve Pubget Overpricing