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2016 ; 17
(1
): 111
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
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English Wikipedia
Taxonomer: an interactive metagenomics analysis portal for universal pathogen
detection and host mRNA expression profiling
#MMPMID27224977
Flygare S
; Simmon K
; Miller C
; Qiao Y
; Kennedy B
; Di Sera T
; Graf EH
; Tardif KD
; Kapusta A
; Rynearson S
; Stockmann C
; Queen K
; Tong S
; Voelkerding KV
; Blaschke A
; Byington CL
; Jain S
; Pavia A
; Ampofo K
; Eilbeck K
; Marth G
; Yandell M
; Schlaberg R
Genome Biol
2016[May]; 17
(1
): 111
PMID27224977
show ga
BACKGROUND: High-throughput sequencing enables unbiased profiling of microbial
communities, universal pathogen detection, and host response to infectious
diseases. However, computation times and algorithmic inaccuracies have hindered
adoption. RESULTS: We present Taxonomer, an ultrafast, web-tool for comprehensive
metagenomics data analysis and interactive results visualization. Taxonomer is
unique in providing integrated nucleotide and protein-based classification and
simultaneous host messenger RNA (mRNA) transcript profiling. Using real-world
case-studies, we show that Taxonomer detects previously unrecognized infections
and reveals antiviral host mRNA expression profiles. To facilitate data-sharing
across geographic distances in outbreak settings, Taxonomer is publicly available
through a web-based user interface. CONCLUSIONS: Taxonomer enables rapid,
accurate, and interactive analyses of metagenomics data on personal computers and
mobile devices.