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10.1002/jcb.25081 http://scihub22266oqcxt.onion/10.1002/jcb.25081 C4880354!4880354!25581900     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Biochem 2015 ; 116 (7): 1165-70 Nephropedia Template TP {{tp|p=25581900|t=2015. Pyrosequencing for Accurate Imprinted Allele Expression Analysis |pdf=|usr=}}{{25581900}} gab.com Text Pyrosequencing for Accurate Imprinted Allele Expression Analysis JO: J Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=25581900 #FOAvip Genomic imprinting is an epigenetic mechanism that restricts gene expression to one inherited allele. Improper maintenance of imprinting has been implicated in a number of human diseases and developmental syndromes. Assays are needed that can quantify the contribution of each paternal allele to a gene expression profile. We have developed a rapid, sensitive quantitative assay for the measurement of individual allelic ratios termed Pyrosequencing for Imprinted Expression (PIE). Advantages of PIE over other approaches include shorter experimental time, decreased labor, avoiding the need for restriction endonuclease enzymes at polymorphic sites, and prevent heteroduplex formation which is problematic in quantitative PCR-based methods. We demonstrate the improved sensitivity of PIE including the ability to detect differences in allelic expression down to 1%. The assay is capable of measuring genomic heterozygosity as well as imprinting in a single run. PIE is applied to determine the status of Insulin-like Growth Factor-2 (IGF2) imprinting in human and mouse tissues. Twit Text FOAVip Pyrosequencing for Accurate Imprinted Allele Expression Analysis ????J Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=25581900 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25581900 #FOAvip English Wikipedia <ref>{{Cite pmid|25581900}}</ref> Pyrosequencing for Accurate Imprinted Allele Expression Analysis #MMPMID25581900 Yang B; Damaschke N; Yao T; McCormick J; Wagner J; Jarrard D J Cell Biochem 2015[Jul]; 116 (7): 1165-70 PMID25581900show ga Genomic imprinting is an epigenetic mechanism that restricts gene expression to one inherited allele. Improper maintenance of imprinting has been implicated in a number of human diseases and developmental syndromes. Assays are needed that can quantify the contribution of each paternal allele to a gene expression profile. We have developed a rapid, sensitive quantitative assay for the measurement of individual allelic ratios termed Pyrosequencing for Imprinted Expression (PIE). Advantages of PIE over other approaches include shorter experimental time, decreased labor, avoiding the need for restriction endonuclease enzymes at polymorphic sites, and prevent heteroduplex formation which is problematic in quantitative PCR-based methods. We demonstrate the improved sensitivity of PIE including the ability to detect differences in allelic expression down to 1%. The assay is capable of measuring genomic heterozygosity as well as imprinting in a single run. PIE is applied to determine the status of Insulin-like Growth Factor-2 (IGF2) imprinting in human and mouse tissues. äPyrosequencing for Accurate Imprinted Allele Expression Analysis (epmc).pdf DeepDyve Pubget Overpricing