Structural Biology of the Proteasome #MMPMID23414347
Kish-Trier E; Hill CP
Annu Rev Biophys 2013[]; 42 (ä): 29-49 PMID23414347show ga
The proteasome refers to a collection of complexes centered on the 20S proteasome core particle, a complex of 28 subunits that houses proteolytic sites in its hollow interior. Proteasomes are found in eukaryotes, archaea, and some eubacteria, and their activity is critical for many cellular pathways. Important advances include inhibitor binding studies and the structure of the immunoproteasome, whose specificity is altered by incorporation of inducible catalytic subunits. The inherent repression of the 20S CP is relieved by the ATP-independent activators, 11S and Blm10/PA200, whose structures reveal principles of proteasome mechanism. The structure of the ATP-dependent 19S regulatory particle, which mediates degradation of polyubiquitylated proteins, is being revealed by a combination of crystal or NMR structures of individual subunits and electron microscopy reconstruction of the intact complex. Other recent structural advances inform about mechanisms of assembly and the role of conformational changes in the functional cycle.
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Annu+Rev+Biophys 2013 ; 42 (ä): 29-49
