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Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Methods+Cell+Biol 2015 ; 130 (ä): 157-80 Nephropedia Template TP
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Methods for analyzing the role of phospholipase A2 enzymes in endosome membrane tubule formation #MMPMID26360034
Kalkofen DN; de Figueiredo P; Brown WJ
Methods Cell Biol 2015[]; 130 (ä): 157-80 PMID26360034show ga
Cargo export from mammalian endosomal compartments often involves membrane tubules, into which soluble and membrane-bound cargos are segregated for subsequent intracellular transport. These membrane tubules are highly dynamic and their formation is mediated by a variety of endosome-associated proteins. However, little is known about how these membrane tubules are temporally or spatially regulated, so other tubule-associated proteins are likely to be discovered and analyzed. Therefore, methods to examine the biogenesis and regulation of endosome membrane tubules will prove to be valuable for cell biologists. In this chapter, we describe methods for studying this process using both cell-free, in vitro reconstitution assays, and in vivo image analysis tools.