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10.1038/srep26463 http://scihub22266oqcxt.onion/10.1038/srep26463 C4876464!4876464 !27212469     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27212469 &cmd=llinks): Failed to open stream: HTTP request failed! 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Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy |pdf=|usr=}}{{27212469 }} gab.com Text Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27212469 #FOAvip The ability to use mesenchymal stromal cells (MSC) directly out of cryostorage would significantly reduce the logistics of MSC therapy by allowing on-site cryostorage of therapeutic doses of MSC at hospitals and clinics. Such a paradigm would be especially advantageous for the treatment of acute conditions such as stroke and myocardial infarction, which are likely to require treatment within hours after ischemic onset. Recently, several reports have emerged that suggest MSC viability and potency are damaged by cryopreservation. Herein we examine the effect of cryopreservation on human MSC viability, immunomodulatory potency, growth factor secretion, and performance in an ischemia/reperfusion injury model. Using modifications of established cryopreservation methods we developed MSC that retain >95% viability upon thawing, remain responsive to inflammatory signals, and are able to suppress activated human peripheral blood mononuclear cells. Most importantly, when injected into the eyes of mice 3?hours after the onset of ischemia and 2?hours after the onset of reperfusion, cryopreserved performed as well as fresh MSC to rescue retinal ganglion cells. Thus, our data suggests when viability is maintained throughout the cryopreservation process, MSC retain their therapeutic potency in both in vitro potency assays and an in vivo ischemia/reperfusion model. Twit Text FOAVip Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27212469 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27212469 #FOAvip English Wikipedia <ref>{{Cite pmid|27212469 }}</ref> Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy #MMPMID27212469 Gramlich OW ; Burand AJ ; Brown AJ ; Deutsch RJ ; Kuehn MH ; Ankrum JA Sci Rep 2016[May]; 6 (ä): 26463 PMID27212469 show ga The ability to use mesenchymal stromal cells (MSC) directly out of cryostorage would significantly reduce the logistics of MSC therapy by allowing on-site cryostorage of therapeutic doses of MSC at hospitals and clinics. Such a paradigm would be especially advantageous for the treatment of acute conditions such as stroke and myocardial infarction, which are likely to require treatment within hours after ischemic onset. Recently, several reports have emerged that suggest MSC viability and potency are damaged by cryopreservation. Herein we examine the effect of cryopreservation on human MSC viability, immunomodulatory potency, growth factor secretion, and performance in an ischemia/reperfusion injury model. Using modifications of established cryopreservation methods we developed MSC that retain >95% viability upon thawing, remain responsive to inflammatory signals, and are able to suppress activated human peripheral blood mononuclear cells. Most importantly, when injected into the eyes of mice 3?hours after the onset of ischemia and 2?hours after the onset of reperfusion, cryopreserved performed as well as fresh MSC to rescue retinal ganglion cells. Thus, our data suggests when viability is maintained throughout the cryopreservation process, MSC retain their therapeutic potency in both in vitro potency assays and an in vivo ischemia/reperfusion model. |Animals [MESH] |Cell Proliferation [MESH] |Cell Survival [MESH] |Cells, Cultured [MESH] |Cryopreservation/*methods [MESH] |Disease Models, Animal [MESH] |Humans [MESH] |Mesenchymal Stem Cell Transplantation/*methods [MESH] |Mesenchymal Stem Cells/*cytology [MESH] |Mice [MESH] |Reperfusion Injury/*therapy [MESH] |Retinal Diseases/*therapy [MESH]Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal (epmc).pdf DeepDyve Pubget Overpricing