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10.1038/srep25516 http://scihub22266oqcxt.onion/10.1038/srep25516 C4876460!4876460!27210050     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2016 ; 6 (ä): ä Nephropedia Template TP {{tp|p=27210050|t=2016. CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization |pdf=|usr=}}{{27210050}} gab.com Text CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27210050 #FOAvip The CRISPR system holds much promise for successful genome engineering, but therapeutic, industrial, and research applications will place high demand on improving the specificity and efficiency of this tool. CT-Finder (http://bioinfolab.miamioh.edu/ct-finder) is a web service to help users design guide RNAs (gRNAs) optimized for specificity. CT-Finder accommodates the original single-gRNA Cas9 system and two specificity-enhancing paired-gRNA systems: Cas9 D10A nickases (Cas9n) and dimeric RNA-guided FokI nucleases (RFNs). Optimal target candidates can be chosen based on the minimization of predicted off-target effects. Graphical visualization of on-target and off-target sites in the genome is provided for target validation. Major model organisms are covered by this web service. Twit Text FOAVip CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27210050 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27210050 #FOAvip English Wikipedia <ref>{{Cite pmid|27210050}}</ref> CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization #MMPMID27210050 Zhu H; Misel L; Graham M; Robinson ML; Liang C Sci Rep 2016[]; 6 (ä): ä PMID27210050show ga The CRISPR system holds much promise for successful genome engineering, but therapeutic, industrial, and research applications will place high demand on improving the specificity and efficiency of this tool. CT-Finder (http://bioinfolab.miamioh.edu/ct-finder) is a web service to help users design guide RNAs (gRNAs) optimized for specificity. CT-Finder accommodates the original single-gRNA Cas9 system and two specificity-enhancing paired-gRNA systems: Cas9 D10A nickases (Cas9n) and dimeric RNA-guided FokI nucleases (RFNs). Optimal target candidates can be chosen based on the minimization of predicted off-target effects. Graphical visualization of on-target and off-target sites in the genome is provided for target validation. Major model organisms are covered by this web service. äCT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visu(epmc).pdf DeepDyve Pubget Overpricing