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10.1038/srep26516

http://scihub22266oqcxt.onion/10.1038/srep26516
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C4876401!4876401!27212232
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suck abstract from ncbi


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pmid27212232      Sci+Rep 2016 ; 6 (ä): ä
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  • The scanning electron microscope in microbiology and diagnosis of infectious disease #MMPMID27212232
  • Golding CG; Lamboo LL; Beniac DR; Booth TF
  • Sci Rep 2016[]; 6 (ä): ä PMID27212232show ga
  • Despite being an excellent tool for investigating ultrastructure, scanning electron microscopy (SEM) is less frequently used than transmission electron microscopy for microbes such as viruses or bacteria. Here we describe rapid methods that allow SEM imaging of fully hydrated, unfixed microbes without using conventional sample preparation methods. We demonstrate improved ultrastructural preservation, with greatly reduced dehydration and shrinkage, for specimens including bacteria and viruses such as Ebola virus using infiltration with ionic liquid on conducting filter substrates for SEM.
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