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2016 ; 10
(3
): 034109
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Micro electrical impedance spectroscopy on a needle for ex vivo discrimination
between human normal and cancer renal tissues
#MMPMID27279933
Yun J
; Kim HW
; Park Y
; Cha JJ
; Lee JZ
; Shin DG
; Lee JH
Biomicrofluidics
2016[May]; 10
(3
): 034109
PMID27279933
show ga
The ex-vivo discrimination between human normal and cancer renal tissues was
confirmed using ?EoN (micro electrical impedance spectroscopy-on-a-needle) by
measuring and comparing the electrical impedances in the frequency domain. To
quantify the extent of discrimination between dissimilar tissues and to determine
the optimal frequency at which the discrimination capability is at a maximum,
discrimination index (DI) was employed for both magnitude and phase. The highest
values of DI for the magnitude and phase were 5.15 at 1?MHz and 3.57 at 1?kHz,
respectively. The mean magnitude and phase measured at the optimal frequency for
normal tissues were 5013.40?±?94.39 ? and -68.54?±?0.72°, respectively; those for
cancer tissues were 4165.19 ±?70.32 ? and -64.10 ±?0.52°, respectively. A
statistically significant difference (p0.05) between the two tissues was
observed at all the investigated frequencies. To extract the electrical
properties (resistance and capacitance) of these bio-tissues through curve
fitting with experimental results, an equivalent circuit was proposed based on
the ?EoN structure on the condition that the ?EoN was immersed in the
bio-tissues. The average and standard deviation of the extracted resistance and
capacitance for the normal tissues were 6.22 ±?0.24?k? and 280.21 ±?32.25?pF,
respectively, and those for the cancer tissues were 5.45?±?0.22?k? and
376.32?±?34.14?pF, respectively. The electrical impedance was higher in the
normal tissues compared with the cancer tissues. The ?EoN could clearly
discriminate between normal and cancer tissues by comparing the results at the
optimal frequency (magnitude and phase) and those of the curve fitting (extracted
resistance and capacitance).