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10.1021/acs.analchem.6b00381 http://scihub22266oqcxt.onion/10.1021/acs.analchem.6b00381 C4872636!4872636!27081872     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chem 2016 ; 88 (10): 5053-7 Nephropedia Template TP {{tp|p=27081872|t=2016. RNA Extraction from a Mycobacterium under Ultrahigh Electric Field Intensity in a Microfluidic Device |pdf=|usr=}}{{27081872}} gab.com Text RNA Extraction from a Mycobacterium under Ultrahigh Electric Field Intensity in a Microfluidic Device JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=27081872 #FOAvip Studies of transcriptomes are critical for understanding gene expression. Release of RNA molecules from cells is typically the first step for transcriptomic analysis. Effective cell lysis approaches that completely release intracellular materials are in high demand especially for cells that are structurally robust. In this report, we demonstrate a microfluidic electric lysis device that is effective for mRNA extraction from mycobacteria that have hydrophobic and waxy cell walls. We used a packed bed of microscale silica beads to filter M. smegmatis out of the suspension. 4000?8000 V/cm field intensity was used to lyse M. smegmatis with long pulses (i.e., up to 30 pulses that were 5 s long each). Our quantitative reverse transcription (qRT)-PCR results showed that our method yielded a factor of 10?20 higher extraction efficiency than the current state-of-the-art method (bead beating). We conclude that our electric lysis technique is an effective approach for mRNA release from hard-to-lyse cells and highly compatible with microfluidic molecular assays. Twit Text FOAVip RNA Extraction from a Mycobacterium under Ultrahigh Electric Field Intensity in a Microfluidic Device ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=27081872 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27081872 #FOAvip English Wikipedia <ref>{{Cite pmid|27081872}}</ref> RNA Extraction from a Mycobacterium under Ultrahigh Electric Field Intensity in a Microfluidic Device #MMPMID27081872 Ma S; Bryson BD; Sun C; Fortune S; Lu C Anal Chem 2016[May]; 88 (10): 5053-7 PMID27081872show ga Studies of transcriptomes are critical for understanding gene expression. Release of RNA molecules from cells is typically the first step for transcriptomic analysis. Effective cell lysis approaches that completely release intracellular materials are in high demand especially for cells that are structurally robust. In this report, we demonstrate a microfluidic electric lysis device that is effective for mRNA extraction from mycobacteria that have hydrophobic and waxy cell walls. We used a packed bed of microscale silica beads to filter M. smegmatis out of the suspension. 4000?8000 V/cm field intensity was used to lyse M. smegmatis with long pulses (i.e., up to 30 pulses that were 5 s long each). Our quantitative reverse transcription (qRT)-PCR results showed that our method yielded a factor of 10?20 higher extraction efficiency than the current state-of-the-art method (bead beating). We conclude that our electric lysis technique is an effective approach for mRNA release from hard-to-lyse cells and highly compatible with microfluidic molecular assays. äRNA Extraction from a Mycobacterium under Ultrahigh Electric Field Int(epmc).pdf DeepDyve Pubget Overpricing