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10.1002/0471250953.bi0508s52

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C4871818!4871818!26678386
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suck abstract from ncbi


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pmid26678386      Curr+Protoc+Bioinformatics 2015 ; 52 (ä): 5.8.1-15
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  • Protein Structure and Function Prediction Using I-TASSER #MMPMID26678386
  • Yang J; Zhang Y
  • Curr Protoc Bioinformatics 2015[]; 52 (ä): 5.8.1-15 PMID26678386show ga
  • I-TASSER is a hierarchical protocol for automated protein structure prediction and structure-based function annotation. Starting from the amino acid sequence of target proteins, I-TASSER first generates full-length atomic structural models from multiple threading alignments and iterative structural assembly simulations followed by atomic-level structure refinement. The biological functions of the protein, including ligand-binding sites, enzyme commission number, and gene ontology terms, are then inferred from known protein function databases based on sequence and structure profile comparisons. I-TASSER is freely available as both an on-line server and a stand-alone package. This unit describes how to use the I-TASSER protocol to generate structure and function prediction and how to interpret the prediction results, as well as alternative approaches for further improving the I-TASSER modeling quality for distant-homologous and multi-domain protein targets.
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