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10.1364/BOE.7.001853

http://scihub22266oqcxt.onion/10.1364/BOE.7.001853
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C4871086!4871086!27231626
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suck abstract from ncbi


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pmid27231626      Biomed+Opt+Express 2016 ; 7 (5): 1853-64
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  • Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques #MMPMID27231626
  • D?Arco A; Brancati N; Ferrara MA; Indolfi M; Frucci M; Sirleto L
  • Biomed Opt Express 2016[May]; 7 (5): 1853-64 PMID27231626show ga
  • The visualization of heterogeneous morphology, segmentation and quantification of image features is a crucial point for nonlinear optics microscopy applications, spanning from imaging of living cells or tissues to biomedical diagnostic. In this paper, a methodology combining stimulated Raman scattering microscopy and image analysis technique is presented. The basic idea is to join the potential of vibrational contrast of stimulated Raman scattering and the strength of imaging analysis technique in order to delineate subcellular morphology with chemical specificity. Validation tests on label free imaging of polystyrene-beads and of adipocyte cells are reported and discussed.
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