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10.1038/ncomms11491 http://scihub22266oqcxt.onion/10.1038/ncomms11491 C4869170!4869170!27173435     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=27173435|t=2016. An organelle-specific protein landscape identifies novel diseases and molecular mechanisms |pdf=|usr=}}{{27173435}} gab.com Text An organelle-specific protein landscape identifies novel diseases and molecular mechanisms JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27173435 #FOAvip Cellular organelles provide opportunities to relate biological mechanisms to disease. Here we use affinity proteomics, genetics and cell biology to interrogate cilia: poorly understood organelles, where defects cause genetic diseases. Two hundred and seventeen tagged human ciliary proteins create a final landscape of 1,319 proteins, 4,905 interactions and 52 complexes. Reverse tagging, repetition of purifications and statistical analyses, produce a high-resolution network that reveals organelle-specific interactions and complexes not apparent in larger studies, and links vesicle transport, the cytoskeleton, signalling and ubiquitination to ciliary signalling and proteostasis. We observe sub-complexes in exocyst and intraflagellar transport complexes, which we validate biochemically, and by probing structurally predicted, disruptive, genetic variants from ciliary disease patients. The landscape suggests other genetic diseases could be ciliary including 3M syndrome. We show that 3M genes are involved in ciliogenesis, and that patient fibroblasts lack cilia. Overall, this organelle-specific targeting strategy shows considerable promise for Systems Medicine. Twit Text FOAVip An organelle-specific protein landscape identifies novel diseases and molecular mechanisms ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=27173435 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27173435 #FOAvip English Wikipedia <ref>{{Cite pmid|27173435}}</ref> An organelle-specific protein landscape identifies novel diseases and molecular mechanisms #MMPMID27173435 Boldt K; van Reeuwijk J; Lu Q; Koutroumpas K; Nguyen TMT; Texier Y; van Beersum SEC; Horn N; Willer JR; Mans DA; Dougherty G; Lamers IJC; Coene KLM; Arts HH; Betts MJ; Beyer T; Bolat E; Gloeckner CJ; Haidari K; Hetterschijt L; Iaconis D; Jenkins D; Klose F; Knapp B; Latour B; Letteboer SJF; Marcelis CL; Mitic D; Morleo M; Oud MM; Riemersma M; Rix S; Terhal PA; Toedt G; van Dam TJP; de Vrieze E; Wissinger Y; Wu KM; Apic G; Beales PL; Blacque OE; Gibson TJ; Huynen MA; Katsanis N; Kremer H; Omran H; van Wijk E; Wolfrum U; Kepes F; Davis EE; Franco B; Giles RH; Ueffing M; Russell RB; Roepman R Nat Commun 2016[]; 7 (ä): ä PMID27173435show ga Cellular organelles provide opportunities to relate biological mechanisms to disease. Here we use affinity proteomics, genetics and cell biology to interrogate cilia: poorly understood organelles, where defects cause genetic diseases. Two hundred and seventeen tagged human ciliary proteins create a final landscape of 1,319 proteins, 4,905 interactions and 52 complexes. Reverse tagging, repetition of purifications and statistical analyses, produce a high-resolution network that reveals organelle-specific interactions and complexes not apparent in larger studies, and links vesicle transport, the cytoskeleton, signalling and ubiquitination to ciliary signalling and proteostasis. We observe sub-complexes in exocyst and intraflagellar transport complexes, which we validate biochemically, and by probing structurally predicted, disruptive, genetic variants from ciliary disease patients. The landscape suggests other genetic diseases could be ciliary including 3M syndrome. We show that 3M genes are involved in ciliogenesis, and that patient fibroblasts lack cilia. Overall, this organelle-specific targeting strategy shows considerable promise for Systems Medicine. äAn organelle-specific protein landscape identifies novel diseases and (epmc).pdf DeepDyve Pubget Overpricing