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10.1371/journal.pone.0155028 http://scihub22266oqcxt.onion/10.1371/journal.pone.0155028 C4868333!4868333!27182698     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2016 ; 11 (5): ä Nephropedia Template TP {{tp|p=27182698|t=2016. Degradation Parameters from Pulse-Chase Experiments |pdf=|usr=}}{{27182698}} gab.com Text Degradation Parameters from Pulse-Chase Experiments JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27182698 #FOAvip Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that constructively includes pulse length in the analysis of decay patterns and extracts the parameters of the underlying degradation process. We also show how to extract decay parameters reliably from measurements taken during the pulse phase. Twit Text FOAVip Degradation Parameters from Pulse-Chase Experiments ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27182698 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27182698 #FOAvip English Wikipedia <ref>{{Cite pmid|27182698}}</ref> Degradation Parameters from Pulse-Chase Experiments #MMPMID27182698 Sin C; Chiarugi D; Valleriani A PLoS One 2016[]; 11 (5): ä PMID27182698show ga Pulse-chase experiments are often used to study the degradation of macromolecules such as proteins or mRNA. Considerations for the choice of pulse length include the toxicity of the pulse to the cell and maximization of labeling. In the general case of non-exponential decay, varying the length of the pulse results in decay patterns that look different. Analysis of these patterns without consideration to pulse length would yield incorrect degradation parameters. Here we propose a method that constructively includes pulse length in the analysis of decay patterns and extracts the parameters of the underlying degradation process. We also show how to extract decay parameters reliably from measurements taken during the pulse phase. äDegradation Parameters from Pulse-Chase Experiments (epmc).pdf DeepDyve Pubget Overpricing