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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 World+J+Gastrointest+Pathophysiol
2016 ; 7
(2
): 235-41
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Visualization of sphingolipids and phospholipids in the fundic gland mucosa of
human stomach using imaging mass spectrometry
#MMPMID27190696
Kurabe N
; Igarashi H
; Ohnishi I
; Tajima S
; Inoue Y
; Takahashi Y
; Setou M
; Sugimura H
World J Gastrointest Pathophysiol
2016[May]; 7
(2
): 235-41
PMID27190696
show ga
AIM: To analyze the lipid distribution in gastric mucosae. METHODS: Imaging mass
spectrometry (MS) is a useful tool to survey the distribution of biomolecules in
surgical specimens. Here we used the imaging MS apparatus named iMScope to
identify the dominant molecules present in the human gastric mucosa near the
fundic glands. Five gastric specimens were subjected to iMScope analysis. These
specimens were also analyzed by immunohistochemistry using MUC5AC,
H(+)-K(+)-ATPase? Claudin18 antibodies. RESULTS: Three major molecules with m/z
725.5, 780.5, and 782.5 detected in the gastric mucosa were identified as
sphingomyelin (SM) (d18:1/16:0), phosphatidylcholine (PC) (16:0/18:2), and PC
(16:0/18:1), respectively, through MS/MS analyses. Using immunohistological
staining, SM (d18:1/16:0) signals were mainly co-localized with the foveolar
epithelium marker MUC5AC. In contrast, PC (16:0/18:2) signals were observed in
the region testing positive for the fundic gland marker H(+)-K(+)-ATPase?. PC
(16:0/18:1) signals were uniformly distributed throughout the mucosa. CONCLUSION:
Our basic data will contribute to the studies of lipid species in physical and
pathological conditions of the human stomach.
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