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High expression of DDX20 enhances the proliferation and metastatic potential of
prostate cancer cells through the NF-?B pathway
#MMPMID27121695
Chen W
; Zhou P
; Li X
Int J Mol Med
2016[Jun]; 37
(6
): 1551-7
PMID27121695
show ga
DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), a member of the DEAD box
protein family, encodes an RNA helicase. Previous research has revealed that
DDX20 may act as a tumor suppressor in hepatocellular carcinoma and as a tumor
promoter in breast cancer. These conflicting reports prompted us to explore the
role of DDX20 in prostate cancer (PCa). To gain insight into the functions of
DDX20 in PCa, we examined DDX20 expression patterns in a PCa tissue microarray
with 99 PCa tissue samples. The results of immunohistochemical staining revealed
that DDX20 expression is frequently upregulated in PCa tissues compared with that
in the adjacent tissues and further clinicopathological analysis showed that the
expression level of DDX20 closely correlates with tumor size, TNM stage (positive
correlation) and patient prognosis (negative correlation). Both
gain?of- and loss?of?function assays were performed in vitro; the overexpression
of DDX20 enhanced the proliferation and metastatic potential of cancer cells and
this was examined by performing a cell counting kit-8 (CCK-8) assay, wound
healing assay and Transwell migration assay. Furthermore, we found that there is
a positive correlation between the expression of matrix metallopeptidase 9 (MMP9)
and DDX20 expression. These findings led us to examine whether DDX20 may exert
effects through the NF??B pathway. Luciferase reporter assays suggested that
DDX20 altered the activity of NF-?B. Taken together, these findings show that
DDX20 may promote the progression of PCa through the NF-?B pathway.
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