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10.1172/jci.insight.87030 http://scihub22266oqcxt.onion/10.1172/jci.insight.87030 C4863708!4863708!27182557     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       JCI+Insight ä ; 1 (6): ä Nephropedia Template TP {{tp|p=27182557|t=ä. Multiplexed immunofluorescence delineates proteomic cancer cell states associated with metabolism |pdf=|usr=}}{{27182557}} gab.com Text Multiplexed immunofluorescence delineates proteomic cancer cell states associated with metabolism JO: JCI Insight http://www.kidney.de/pget.php?&tw=1&pmid=27182557 #FOAvip The phenotypic diversity of cancer results from genetic and nongenetic factors. Most studies of cancer heterogeneity have focused on DNA alterations, as technologies for proteomic measurements in clinical specimen are currently less advanced. Here, we used a multiplexed immunofluorescence staining platform to measure the expression of 27 proteins at the single-cell level in formalin-fixed and paraffin-embedded samples from treatment-naive stage II/III human breast cancer. Unsupervised clustering of protein expression data from 638,577 tumor cells in 26 breast cancers identified 8 clusters of protein coexpression. In about one-third of breast cancers, over 95% of all neoplastic cells expressed a single protein coexpression cluster. The remaining tumors harbored tumor cells representing multiple protein coexpression clusters, either in a regional distribution or intermingled throughout the tumor. Tumor uptake of the radiotracer 18F-fluorodeoxyglucose was associated with protein expression clusters characterized by hormone receptor loss, PTEN alteration, and HER2 gene amplification. Our study demonstrates an approach to generate cellular heterogeneity metrics in routinely collected solid tumor specimens and integrate them with in vivo cancer phenotypes. Twit Text FOAVip Multiplexed immunofluorescence delineates proteomic cancer cell states associated with metabolism ????JCI Insight http://www.kidney.de/pget.php?&tw=1&pmid=27182557 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27182557 #FOAvip English Wikipedia <ref>{{Cite pmid|27182557}}</ref> Multiplexed immunofluorescence delineates proteomic cancer cell states associated with metabolism #MMPMID27182557 Sood A; Miller AM; Brogi E; Sui Y; Armenia J; McDonough E; Santamaria-Pang A; Carlin S; Stamper A; Campos C; Pang Z; Li Q; Port E; Graeber TG; Schultz N; Ginty F; Larson SM; Mellinghoff IK JCI Insight ä[]; 1 (6): ä PMID27182557show ga The phenotypic diversity of cancer results from genetic and nongenetic factors. Most studies of cancer heterogeneity have focused on DNA alterations, as technologies for proteomic measurements in clinical specimen are currently less advanced. Here, we used a multiplexed immunofluorescence staining platform to measure the expression of 27 proteins at the single-cell level in formalin-fixed and paraffin-embedded samples from treatment-naive stage II/III human breast cancer. Unsupervised clustering of protein expression data from 638,577 tumor cells in 26 breast cancers identified 8 clusters of protein coexpression. In about one-third of breast cancers, over 95% of all neoplastic cells expressed a single protein coexpression cluster. The remaining tumors harbored tumor cells representing multiple protein coexpression clusters, either in a regional distribution or intermingled throughout the tumor. Tumor uptake of the radiotracer 18F-fluorodeoxyglucose was associated with protein expression clusters characterized by hormone receptor loss, PTEN alteration, and HER2 gene amplification. Our study demonstrates an approach to generate cellular heterogeneity metrics in routinely collected solid tumor specimens and integrate them with in vivo cancer phenotypes. äMultiplexed immunofluorescence delineates proteomic cancer cell states(epmc).pdf DeepDyve Pubget Overpricing