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2016 ; 28
(4
): 875-91
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Three-Dimensional Visualization of the Tubular-Lamellar Transformation of the
Internal Plastid Membrane Network during Runner Bean Chloroplast Biogenesis
#MMPMID27002023
Kowalewska ?
; Mazur R
; Suski S
; Garstka M
; Mostowska A
Plant Cell
2016[Apr]; 28
(4
): 875-91
PMID27002023
show ga
Chloroplast biogenesis is a complex process that is integrated with plant
development, leading to fully differentiated and functionally mature plastids. In
this work, we used electron tomography and confocal microscopy to reconstruct the
process of structural membrane transformation during the etioplast-to-chloroplast
transition in runner bean (Phaseolus coccineus). During chloroplast development,
the regular tubular network of paracrystalline prolamellar bodies (PLBs) and the
flattened porous membranes of prothylakoids develop into the chloroplast
thylakoids. Three-dimensional reconstruction is required to provide us with a
more complete understanding of this transformation. We provide spatial models of
the bean chloroplast biogenesis that allow such reconstruction of the internal
membranes of the developing chloroplast and visualize the transformation from the
tubular arrangement to the linear system of parallel lamellae. We prove that the
tubular structure of the PLB transforms directly to flat slats, without
dispersion to vesicles. We demonstrate that the grana/stroma thylakoid
connections have a helical character starting from the early stages of appressed
membrane formation. Moreover, we point out the importance of particular
chlorophyll-protein complex components in the membrane stacking during the
biogenesis. The main stages of chloroplast internal membrane biogenesis are
presented in a movie that shows the time development of the chloroplast
biogenesis as a dynamic model of this process.