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10.1074/jbc.M115.710533 http://scihub22266oqcxt.onion/10.1074/jbc.M115.710533 C4861475!4861475 !26896002     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26896002 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Biol+Chem 2016 ; 291 (17 ): 9060-72 Nephropedia Template TP {{tp|p=26896002 |t=2016. Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 ?-Aggregated Structures in Influenza A Virus-infected Cells |pdf=|usr=}}{{26896002 }} gab.com Text Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 -Aggregated Structures in Influenza A Virus-infected Cells JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26896002 #FOAvip PB1-F2 is a virulence factor of influenza A virus (IAV) whose functions remain misunderstood. The different roles of PB1-F2 may be linked to its structural polymorphism and to its propensity to assemble into oligomers and amyloid fibers in the vicinity of the membrane of IAV-infected cells. Here, we monitored the impact of PB1-F2 on the biochemical composition and protein structures of human epithelial pulmonary cells (A549) and monocytic cells (U937) upon IAV infection using synchrotron Fourier-transform infrared (FTIR) and deep UV (DUV) microscopies at the single-cell level. Cells were infected with a wild-type IAV and its PB1-F2 knock-out mutant for analyses at different times post-infection. IR spectra were recorded in each condition and processed to evaluate the change in the component band of the spectra corresponding to the amide I (secondary structure) and the CH stretching region (membrane). The IR spectra analysis revealed that expression of PB1-F2 in U937 cells, but not in A549 cells, results in the presence of a specific ?-aggregate signature. Furthermore, the lipid membrane composition of U937 cells expressing PB1-F2 was also altered in a cell type-dependent manner. Using DUV microscopy and taking advantage of the high content of tryptophan residues in the sequence of PB1-F2 (5/90 aa), we showed that the increase of the autofluorescent signal recorded in monocytic cells could be correlated with the IR detection of ?-aggregates. Altogether, our results constitute an important step forward in the understanding of the cell type-dependent function of PB1-F2. Twit Text FOAVip Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 -Aggregated Structures in Influenza A Virus-infected Cells ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=26896002 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26896002 #FOAvip English Wikipedia <ref>{{Cite pmid|26896002 }}</ref> Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study of PB1-F2 ?-Aggregated Structures in Influenza A Virus-infected Cells #MMPMID26896002 Chevalier C ; Le Goffic R ; Jamme F ; Leymarie O ; Réfrégiers M ; Delmas B J Biol Chem 2016[Apr]; 291 (17 ): 9060-72 PMID26896002 show ga PB1-F2 is a virulence factor of influenza A virus (IAV) whose functions remain misunderstood. The different roles of PB1-F2 may be linked to its structural polymorphism and to its propensity to assemble into oligomers and amyloid fibers in the vicinity of the membrane of IAV-infected cells. Here, we monitored the impact of PB1-F2 on the biochemical composition and protein structures of human epithelial pulmonary cells (A549) and monocytic cells (U937) upon IAV infection using synchrotron Fourier-transform infrared (FTIR) and deep UV (DUV) microscopies at the single-cell level. Cells were infected with a wild-type IAV and its PB1-F2 knock-out mutant for analyses at different times post-infection. IR spectra were recorded in each condition and processed to evaluate the change in the component band of the spectra corresponding to the amide I (secondary structure) and the CH stretching region (membrane). The IR spectra analysis revealed that expression of PB1-F2 in U937 cells, but not in A549 cells, results in the presence of a specific ?-aggregate signature. Furthermore, the lipid membrane composition of U937 cells expressing PB1-F2 was also altered in a cell type-dependent manner. Using DUV microscopy and taking advantage of the high content of tryptophan residues in the sequence of PB1-F2 (5/90 aa), we showed that the increase of the autofluorescent signal recorded in monocytic cells could be correlated with the IR detection of ?-aggregates. Altogether, our results constitute an important step forward in the understanding of the cell type-dependent function of PB1-F2. |*Protein Aggregates [MESH] |Cell Membrane/genetics/metabolism/virology [MESH] |HeLa Cells [MESH] |Humans [MESH] |Influenza A Virus, H1N1 Subtype/genetics/*metabolism/*pathogenicity [MESH] |Influenza, Human/genetics/*metabolism [MESH] |Microscopy, Fluorescence [MESH] |U937 Cells [MESH]Synchrotron Infrared and Deep UV Fluorescent Microspectroscopy Study o(epmc).pdf DeepDyve Pubget Overpricing