Detecting PTEN and PI3K signaling in brain #MMPMID27033070
Zhu G; Baker SJ
Methods Mol Biol 2016[]; 1388 (ä): 53-62 PMID27033070show ga
The central nervous system is comprised of multiple cell types including neurons, glia and other supporting cells that may differ dramatically in levels of signaling pathway activation. Immunohistochemistry in conjunction with drug interference are powerful tools that allow evaluation of signaling pathways in different cell types of the mouse central nervous system in vivo. Here we provide detailed protocols for immunohistochemistry to evaluate three essential components in the PI3K pathway in mouse brain: Pten, p-Akt and p-4ebp1, and for rapamycin treatment to modulate mTOR signaling in vivo.