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2011 ; 12
(1
): 65-70
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Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific
protein labeling in living cells
#MMPMID21154801
Jin X
; Uttamapinant C
; Ting AY
Chembiochem
2011[Jan]; 12
(1
): 65-70
PMID21154801
show ga
PRIME time: We report the synthesis of a pH-insensitive blue fluorophore,
7-aminocoumarin, by using palladium-catalyzed Buchwald?Hartwig cross coupling.
7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and
inside living cells through PRIME (probe incorporation mediated by enzymes), and
unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as
endosomes. [Image: see text]