Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1093/nar/gkv1542 http://scihub22266oqcxt.onion/10.1093/nar/gkv1542 C4856974!4856974!26762978     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nucleic+Acids+Res 2016 ; 44 (8): e76 Nephropedia Template TP {{tp|p=26762978|t=2016. In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining |pdf=|usr=}}{{26762978}} gab.com Text In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining JO: Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26762978 #FOAvip The CRISPR/Cas9 system facilitates precise DNA modifications by generating RNA-guided blunt-ended double-strand breaks. We demonstrate that guide RNA pairs generate deletions that are repaired with a high level of precision by non-homologous end-joining in mammalian cells. We present a method called knock-in blunt ligation for exploiting these breaks to insert exogenous PCR-generated sequences in a homology-independent manner without loss of additional nucleotides. This method is useful for making precise additions to the genome such as insertions of marker gene cassettes or functional elements, without the need for homology arms. We successfully utilized this method in human and mouse cells to insert fluorescent protein cassettes into various loci, with efficiencies up to 36% in HEK293 cells without selection. We also created versions of Cas9 fused to the FKBP12-L106P destabilization domain in an effort to improve Cas9 performance. Our in vivo blunt-end cloning method and destabilization-domain-fused Cas9 variant increase the repertoire of precision genome engineering approaches. Twit Text FOAVip In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining ????Nucleic Acids Res http://www.kidney.de/pget.php?&tw=1&pmid=26762978 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26762978 #FOAvip English Wikipedia <ref>{{Cite pmid|26762978}}</ref> In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining #MMPMID26762978 Geisinger JM; Turan S; Hernandez S; Spector LP; Calos MP Nucleic Acids Res 2016[May]; 44 (8): e76 PMID26762978show ga The CRISPR/Cas9 system facilitates precise DNA modifications by generating RNA-guided blunt-ended double-strand breaks. We demonstrate that guide RNA pairs generate deletions that are repaired with a high level of precision by non-homologous end-joining in mammalian cells. We present a method called knock-in blunt ligation for exploiting these breaks to insert exogenous PCR-generated sequences in a homology-independent manner without loss of additional nucleotides. This method is useful for making precise additions to the genome such as insertions of marker gene cassettes or functional elements, without the need for homology arms. We successfully utilized this method in human and mouse cells to insert fluorescent protein cassettes into various loci, with efficiencies up to 36% in HEK293 cells without selection. We also created versions of Cas9 fused to the FKBP12-L106P destabilization domain in an effort to improve Cas9 performance. Our in vivo blunt-end cloning method and destabilization-domain-fused Cas9 variant increase the repertoire of precision genome engineering approaches. äIn vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologo(epmc).pdf DeepDyve Pubget Overpricing