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Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative
and in situ analysis of gangliosides in brain
#MMPMID27142336
Zhang Y
; Wang J
; Liu J
; Han J
; Xiong S
; Yong W
; Zhao Z
Sci Rep
2016[May]; 6
(?): 25289
PMID27142336
show ga
Gangliosides are a family of complex lipids that are abundant in the brain. There
is no doubt the investigations about the distribution of gangliosides in brian
and the relationship between gangliosides and Alzheimer's disease is profound.
However, these investigations are full of challenges due to the structural
complexity of gangliosides. In this work, the method for efficient extraction and
enrichment of gangliosides from brain was established. Moreover, the distribution
of gangliosides in brain was obtained by matrix-assisted laser desorption
ionization (MALDI) mass spectrometry imaging (MSI). It was found that
3-aminoquinoline (3-AQ) as matrix was well-suited for MALDI MS analysis of
gangliosides in negative ion mode. In addition, the pretreatment by ethanol
(EtOH) cleaning brain section and the addition of ammonium formate greatly
improved the MS signal of gangliosides in the brain section when MALDI MSI
analysis was employed. The distribution of ganliosides in cerebral cortex,
hippocampus and cerebellum was respectively acquired by electrospray ionization
(ESI) MS and MALDI MSI, and the data were compared for reliability evaluation of
MALDI MSI. Further, applying MALDI MSI technology, the distribution of
gangliosides in amyloid precursor protein transgenic mouse brain was obtained,
which may provide a new insight for bioresearch of Alzheimer's disease (AD).
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