Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27029011
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Assoc+Res+Otolaryngol
2016 ; 17
(3
): 159-71
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Scanning Electron Microscopic Examination of the Extracellular Matrix in the
Decellularized Mouse and Human Cochlea
#MMPMID27029011
Santi PA
; Aldaya R
; Brown A
; Johnson S
; Stromback T
; Cureoglu S
; Rask-Andersen H
J Assoc Res Otolaryngol
2016[Jun]; 17
(3
): 159-71
PMID27029011
show ga
Decellularized tissues have been used to investigate the extracellular matrix
(ECM) in a number of different tissues and species. Santi and Johnson JARO
14:3-15 (2013) first described the decellularized inner ear in the mouse, rat,
and human using scanning thin-sheet laser imaging microscopy (sTSLIM). The
purpose of the present investigation is to examine decellularized cochleas in the
mouse and human at higher resolution using scanning electron microscopy (SEM).
Fresh cochleas were harvested and decellularized using detergent extraction
methods. Following decellularization, the ECM of the bone, basilar membrane,
spiral limbus, and ligament remained, and all of the cells were removed from the
cochlea. A number of similarities and differences in the ECM of the mouse and
human were observed. A novel, spirally directed structure was present on the
basilar membrane and is located at the border between Hensen and Boettcher cells.
These septa-like structures formed a single row in the mouse and multiple rows in
the human. The basal lamina of the stria vascularis capillaries was present and
appeared thicker in the human compared with the mouse. In the mouse, numerous
openings beneath the spiral prominence that previously housed the root processes
of the external sulcus cells were observed but in the human there was only a
single row of openings. These and other anatomical differences in the ECM between
the mouse and human may reflect functional differences and/or be due to aging;
however, decellularized cochleas provide a new way to examine the cochlear ECM
and reveal new observations.