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10.1371/journal.pone.0154460 http://scihub22266oqcxt.onion/10.1371/journal.pone.0154460 C4852981!4852981 !27136356     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27136356 &cmd=llinks): Failed to open stream: HTTP request failed! 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Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phosphorylation and Actin Polymerization in Human Saphenous Vein |pdf=|usr=}}{{27136356 }} gab.com Text Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phosphorylation and Actin Polymerization in Human Saphenous Vein JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27136356 #FOAvip OBJECTIVE: Papaverine is used to prevent vasospasm in human saphenous veins (HSV) during vein graft preparation prior to implantation as a bypass conduit. Papaverine is a nonspecific inhibitor of phosphodiesterases, leading to increases in both intracellular cGMP and cAMP. We hypothesized that papaverine reduces force by decreasing intracellular calcium concentrations ([Ca2+]i) and myosin light chain phosphorylation, and increasing actin depolymerization via regulation of actin regulatory protein phosphorylation. APPROACH AND RESULTS: HSV was equilibrated in a muscle bath, pre-treated with 1 mM papaverine followed by 5 ?M norepinephrine, and force along with [Ca2+]i levels were concurrently measured. Filamentous actin (F-actin) level was measured by an in vitro actin assay. Tissue was snap frozen to measure myosin light chain and actin regulatory protein phosphorylation. Pre-treatment with papaverine completely inhibited norepinephrine-induced force generation, blocked increases in [Ca2+]i and led to a decrease in the phosphorylation of myosin light chain. Papaverine pre-treatment also led to increased phosphorylation of the heat shock-related protein 20 (HSPB6) and the vasodilator stimulated phosphoprotein (VASP), as well as decreased filamentous actin (F-actin) levels suggesting depolymerization of actin. CONCLUSIONS: These results suggest that papaverine-induced force inhibition of HSV involves [Ca2+]i-mediated inhibition of myosin light chain phosphorylation and actin regulatory protein phosphorylation-mediated actin depolymerization. Thus, papaverine induces sustained inhibition of contraction of HSV by the modulation of both myosin cross-bridge formation and actin cytoskeletal dynamics and is a pharmacological alternative to high pressure distention to prevent vasospasm. Twit Text FOAVip Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phosphorylation and Actin Polymerization in Human Saphenous Vein ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27136356 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27136356 #FOAvip English Wikipedia <ref>{{Cite pmid|27136356 }}</ref> Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phosphorylation and Actin Polymerization in Human Saphenous Vein #MMPMID27136356 Hocking KM ; Putumbaka G ; Wise ES ; Cheung-Flynn J ; Brophy CM ; Komalavilas P PLoS One 2016[]; 11 (5 ): e0154460 PMID27136356 show ga OBJECTIVE: Papaverine is used to prevent vasospasm in human saphenous veins (HSV) during vein graft preparation prior to implantation as a bypass conduit. Papaverine is a nonspecific inhibitor of phosphodiesterases, leading to increases in both intracellular cGMP and cAMP. We hypothesized that papaverine reduces force by decreasing intracellular calcium concentrations ([Ca2+]i) and myosin light chain phosphorylation, and increasing actin depolymerization via regulation of actin regulatory protein phosphorylation. APPROACH AND RESULTS: HSV was equilibrated in a muscle bath, pre-treated with 1 mM papaverine followed by 5 ?M norepinephrine, and force along with [Ca2+]i levels were concurrently measured. Filamentous actin (F-actin) level was measured by an in vitro actin assay. Tissue was snap frozen to measure myosin light chain and actin regulatory protein phosphorylation. Pre-treatment with papaverine completely inhibited norepinephrine-induced force generation, blocked increases in [Ca2+]i and led to a decrease in the phosphorylation of myosin light chain. Papaverine pre-treatment also led to increased phosphorylation of the heat shock-related protein 20 (HSPB6) and the vasodilator stimulated phosphoprotein (VASP), as well as decreased filamentous actin (F-actin) levels suggesting depolymerization of actin. CONCLUSIONS: These results suggest that papaverine-induced force inhibition of HSV involves [Ca2+]i-mediated inhibition of myosin light chain phosphorylation and actin regulatory protein phosphorylation-mediated actin depolymerization. Thus, papaverine induces sustained inhibition of contraction of HSV by the modulation of both myosin cross-bridge formation and actin cytoskeletal dynamics and is a pharmacological alternative to high pressure distention to prevent vasospasm. |Actins/*metabolism [MESH] |Calcium/metabolism [MESH] |Humans [MESH] |Immunoblotting [MESH] |Muscle, Smooth, Vascular/drug effects/metabolism [MESH] |Myosin Light Chains/*metabolism [MESH] |Papaverine/*pharmacology [MESH] |Phosphorylation/drug effects [MESH] |Saphenous Vein/*drug effects/*metabolism [MESH]Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phos(epmc).pdf DeepDyve Pubget Overpricing