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10.1155/2016/6391264 http://scihub22266oqcxt.onion/10.1155/2016/6391264 C4852366!4852366!27195303     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol+Res 2016 ; 2016 (ä): ä Nephropedia Template TP {{tp|p=27195303|t=2016. Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles |pdf=|usr=}}{{27195303}} gab.com Text Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles JO: J Immunol Res http://www.kidney.de/pget.php?&tw=1&pmid=27195303 #FOAvip Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great interest to investigate EVs in more detail with focus on immunological markers. In this study, five major leukocyte subpopulations and the corresponding leukocyte-derived EVs were phenotyped with focus on selected immunological lineage-specific markers and selected vesicle-related markers. The leukocyte-derived EVs displayed phenotypic differences in the 34 markers investigated. The majority of the lineage-specific markers used for identification of the parent cell types could not be detected on EVs released from monocultures of the associated cell types. In contrast, the vesicular presentation of CD9, CD63, and CD81 correlated to the cell surface expression of these markers, however, with few exceptions. Furthermore, the cellular expression of CD9, CD63, and CD81 varied between leukocytes present in whole blood and cultured leukocytes. In summary, these data demonstrate that the cellular and vesicular presentation of selected lineage-specific and vesicle-related markers may differ, supporting the accumulating observations that sorting of molecular cargo into EVs is tightly controlled. Twit Text FOAVip Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles ????J Immunol Res http://www.kidney.de/pget.php?&tw=1&pmid=27195303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27195303 #FOAvip English Wikipedia <ref>{{Cite pmid|27195303}}</ref> Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles #MMPMID27195303 Pugholm LH; Bæk R; Søndergaard EKL; Revenfeld ALS; Jørgensen MM; Varming K J Immunol Res 2016[]; 2016 (ä): ä PMID27195303show ga Extracellular vesicles (EVs) have a demonstrated involvement in modulating the immune system. It has been proposed that EVs could be used as biomarkers for detection of inflammatory and immunological disorders. Consequently, it is of great interest to investigate EVs in more detail with focus on immunological markers. In this study, five major leukocyte subpopulations and the corresponding leukocyte-derived EVs were phenotyped with focus on selected immunological lineage-specific markers and selected vesicle-related markers. The leukocyte-derived EVs displayed phenotypic differences in the 34 markers investigated. The majority of the lineage-specific markers used for identification of the parent cell types could not be detected on EVs released from monocultures of the associated cell types. In contrast, the vesicular presentation of CD9, CD63, and CD81 correlated to the cell surface expression of these markers, however, with few exceptions. Furthermore, the cellular expression of CD9, CD63, and CD81 varied between leukocytes present in whole blood and cultured leukocytes. In summary, these data demonstrate that the cellular and vesicular presentation of selected lineage-specific and vesicle-related markers may differ, supporting the accumulating observations that sorting of molecular cargo into EVs is tightly controlled. äPhenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles(epmc).pdf DeepDyve Pubget Overpricing