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10.1371/journal.pone.0154604 http://scihub22266oqcxt.onion/10.1371/journal.pone.0154604 C4851410!4851410!27128441     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2016 ; 11 (4): ä Nephropedia Template TP {{tp|p=27128441|t=2016. Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9 |pdf=|usr=}}{{27128441}} gab.com Text Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9 JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27128441 #FOAvip The mammalian prion protein (PrP, encoded by Prnp) is most infamous for its central role in prion diseases, invariably fatal neurodegenerative diseases affecting humans, food animals, and animals in the wild. However, PrP is also hypothesized to be an important receptor for toxic protein conformers in Alzheimer's disease, and is associated with other clinically relevant processes such as cancer and stroke. Thus, key insights into important clinical areas, as well as into understanding PrP functions in normal physiology, can be obtained from studying transgenic mouse models and cell culture systems. However, the Prnp locus is difficult to manipulate by homologous recombination, making modifications of the endogenous locus rarely attempted. Fortunately in recent years genome engineering technologies, like TALENs or CRISPR/Cas9 (CC9), have brought exceptional new possibilities for manipulating Prnp. Herein, we present our observations made during systematic experiments with the CC9 system targeting the endogenous mouse Prnp locus, to either modify sequences or to boost PrP expression using CC9-based synergistic activation mediators (SAMs). It is our hope that this information will aid and encourage researchers to implement gene-targeting techniques into their research program. Twit Text FOAVip Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9 ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27128441 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27128441 #FOAvip English Wikipedia <ref>{{Cite pmid|27128441}}</ref> Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9 #MMPMID27128441 Kaczmarczyk L; Mende Y; Zevnik B; Jackson WS PLoS One 2016[]; 11 (4): ä PMID27128441show ga The mammalian prion protein (PrP, encoded by Prnp) is most infamous for its central role in prion diseases, invariably fatal neurodegenerative diseases affecting humans, food animals, and animals in the wild. However, PrP is also hypothesized to be an important receptor for toxic protein conformers in Alzheimer's disease, and is associated with other clinically relevant processes such as cancer and stroke. Thus, key insights into important clinical areas, as well as into understanding PrP functions in normal physiology, can be obtained from studying transgenic mouse models and cell culture systems. However, the Prnp locus is difficult to manipulate by homologous recombination, making modifications of the endogenous locus rarely attempted. Fortunately in recent years genome engineering technologies, like TALENs or CRISPR/Cas9 (CC9), have brought exceptional new possibilities for manipulating Prnp. Herein, we present our observations made during systematic experiments with the CC9 system targeting the endogenous mouse Prnp locus, to either modify sequences or to boost PrP expression using CC9-based synergistic activation mediators (SAMs). It is our hope that this information will aid and encourage researchers to implement gene-targeting techniques into their research program. äManipulating the Prion Protein Gene Sequence and Expression Levels wit(epmc).pdf DeepDyve Pubget Overpricing