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10.1186/s12860-016-0101-0 http://scihub22266oqcxt.onion/10.1186/s12860-016-0101-0 C4850690!4850690!27130612     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27130612.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       BMC+Cell+Biol 2016 ; 17 (ä): ä Nephropedia Template TP {{tp|p=27130612|t=2016. Matrix metalloproteinase 9 induces endothelial-mesenchymal transition via Notch activation in human kidney glomerular endothelial cells |pdf=|usr=}}{{27130612}} gab.com Text Matrix metalloproteinase 9 induces endothelial-mesenchymal transition via Notch activation in human kidney glomerular endothelial cells JO: BMC Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=27130612 #FOAvip Background: Endothelial-mesenchymal transition (EndoMT) is a major source of myofibroblast formation in kidney fibrosis. Our previous study showed a profibrotic role for matrix metalloproteinase 9 (MMP-9) in kidney fibrosis via induction of epithelial-mesenchymal transition (EMT). Inhibition of MMP-9 activity reduced kidney fibrosis in murine unilateral ureteral obstruction. This study investigated whether MMP-9 also plays a role in EndoMT in human glomerular endothelial cells. Results: TGF-?1 (10 or 20 ng/ml) induced EndoMT in HKGECs as shown by morphological changes. In addition, VE-cadherin and CD31 were significantly downregulated, whereas ?-SMA, vimentin, and N-cadherin were upregulated. RT-PCR revealed that Snail, a known inducer of EMT, was upregulated. The MMP inhibitor GM6001 abrogated TGF-?1-induced EndoMT. Zymography indicated that MMP-9 was also upregulated in TGF-?1-treated HKGECs. Recombinant MMP-9 (2 ?g/ml) induced EndoMT in HKGECs via Notch signaling, as evidenced by increased formation of the Notch intracellular domain (NICD) and decreased Notch 1. Inhibition of MMP-9 activity by its inhibitor showed a dose-dependent response in preventing TGF-?1-induced ?-SMA and NICD in HKGECs, whereas inhibition of Notch signaling by ?-secretase inhibitor (GSI) blocked rMMP-9-induced EndoMT. Conclusions: Taken together, our results demonstrate that MMP-9 plays an important role in TGF-?1-induced EndoMT via upregulation of Notch signaling in HKGECs. Twit Text FOAVip Matrix metalloproteinase 9 induces endothelial-mesenchymal transition via Notch activation in human kidney glomerular endothelial cells ????BMC Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=27130612 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27130612 #FOAvip English Wikipedia <ref>{{Cite pmid|27130612}}</ref> Matrix metalloproteinase 9 induces endothelial-mesenchymal transition via Notch activation in human kidney glomerular endothelial cells #MMPMID27130612 Zhao Y; Qiao X; Wang L; Tan TK; Zhao H; Zhang Y; Zhang J; Rao P; Cao Q; Wang Y; Wang Y; Wang YM; Lee VWS; Alexander SI; Harris DCH; Zheng G BMC Cell Biol 2016[]; 17 (ä): ä PMID27130612show ga Background: Endothelial-mesenchymal transition (EndoMT) is a major source of myofibroblast formation in kidney fibrosis. Our previous study showed a profibrotic role for matrix metalloproteinase 9 (MMP-9) in kidney fibrosis via induction of epithelial-mesenchymal transition (EMT). Inhibition of MMP-9 activity reduced kidney fibrosis in murine unilateral ureteral obstruction. This study investigated whether MMP-9 also plays a role in EndoMT in human glomerular endothelial cells. Results: TGF-?1 (10 or 20 ng/ml) induced EndoMT in HKGECs as shown by morphological changes. In addition, VE-cadherin and CD31 were significantly downregulated, whereas ?-SMA, vimentin, and N-cadherin were upregulated. RT-PCR revealed that Snail, a known inducer of EMT, was upregulated. The MMP inhibitor GM6001 abrogated TGF-?1-induced EndoMT. Zymography indicated that MMP-9 was also upregulated in TGF-?1-treated HKGECs. Recombinant MMP-9 (2 ?g/ml) induced EndoMT in HKGECs via Notch signaling, as evidenced by increased formation of the Notch intracellular domain (NICD) and decreased Notch 1. Inhibition of MMP-9 activity by its inhibitor showed a dose-dependent response in preventing TGF-?1-induced ?-SMA and NICD in HKGECs, whereas inhibition of Notch signaling by ?-secretase inhibitor (GSI) blocked rMMP-9-induced EndoMT. Conclusions: Taken together, our results demonstrate that MMP-9 plays an important role in TGF-?1-induced EndoMT via upregulation of Notch signaling in HKGECs. äMatrix metalloproteinase 9 induces endothelial-mesenchymal transition (epmc).pdf DeepDyve Pubget Overpricing