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10.1016/j.ymeth.2016.01.010 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2016.01.010 C4848123!4848123!26804563     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26804563&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Methods 2016 ; 100 (ä): 16-24 Nephropedia Template TP {{tp|p=26804563|t=2016. Antibody-based affinity cryo-EM grid |pdf=|usr=}}{{26804563}} gab.com Text Antibody-based affinity cryo-EM grid JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=26804563 #FOAvip The Affinity Grid technique combines sample purification and cryo-Electron Microscopy (cryo-EM) grid preparation into a single step. Several types of affinity surfaces, including functionalized lipids monolayers, streptavidin 2D crystals, and covalently functionalized carbon surfaces have been reported. More recently, we presented a new affinity cryo-EM approach, cryo-SPIEM, which applies the traditional Solid Phase Immune Electron Microscopy (SPIEM) technique to cryo-EM. This approach significantly simplifies the preparation of affinity grids and directly works with native macromolecular complexes without need of target modifications. With wide availability of high affinity and high specificity antibodies, the antibody-based affinity grid would enable cryo-EM studies of the native samples directly from cell cultures, targets of low abundance, and unstable or short-lived intermediate states. Twit Text FOAVip Antibody-based affinity cryo-EM grid ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=26804563 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26804563 #FOAvip English Wikipedia <ref>{{Cite pmid|26804563}}</ref> Antibody-based affinity cryo-EM grid #MMPMID26804563 Yu G; Li K; Jiang W Methods 2016[May]; 100 (ä): 16-24 PMID26804563show ga The Affinity Grid technique combines sample purification and cryo-Electron Microscopy (cryo-EM) grid preparation into a single step. Several types of affinity surfaces, including functionalized lipids monolayers, streptavidin 2D crystals, and covalently functionalized carbon surfaces have been reported. More recently, we presented a new affinity cryo-EM approach, cryo-SPIEM, which applies the traditional Solid Phase Immune Electron Microscopy (SPIEM) technique to cryo-EM. This approach significantly simplifies the preparation of affinity grids and directly works with native macromolecular complexes without need of target modifications. With wide availability of high affinity and high specificity antibodies, the antibody-based affinity grid would enable cryo-EM studies of the native samples directly from cell cultures, targets of low abundance, and unstable or short-lived intermediate states. äAntibody-based affinity cryo-EM grid (epmc).pdf DeepDyve Pubget Overpricing