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2016 ; 57
(5
): 858-67
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In-depth sphingomyelin characterization using electron impact excitation of ions
from organics and mass spectrometry
#MMPMID27005317
Baba T
; Campbell JL
; Le Blanc JC
; Baker PR
J Lipid Res
2016[May]; 57
(5
): 858-67
PMID27005317
show ga
Electron impact excitation of ions from organics (EIEIO), also referred to as
electron-induced dissociation, was applied to singly charged SM molecular species
in the gas phase. Using ESI and a quadrupole TOF mass spectrometer equipped with
an electron-ion reaction device, we found that SMs fragmented sufficiently to
identify their lipid class, acyl group structure, and the location of double
bond(s). Using this technique, nearly 200 SM molecular species were found in four
natural lipid extracts: bovine milk, porcine brain, chicken egg yolk, and bovine
heart. In addition to the most common backbone, d18:1, sphingosines with a range
of carbon chain lengths, sphingadienes, and some sphinganine backbones were also
detected. Modifications in natural SMs were also identified, including addition
of iodine/methanol across a carbon-carbon double bond. This unparalleled new
approach to SM analysis using EIEIO-MS shows promise as a unique and powerful
tool for structural characterization.