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2016 ; 8
(2
): 1155-63
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MicroRNA-223-3p suppresses leukemia inhibitory factor expression and pinopodes
formation during embryo implantation in mice
#MMPMID27158401
Dong X
; Sui C
; Huang K
; Wang L
; Hu D
; Xiong T
; Wang R
; Zhang H
Am J Transl Res
2016[]; 8
(2
): 1155-63
PMID27158401
show ga
MicroRNA (miRNA) regulates gene expression in a post-transcriptional manner,
which hybridizes the target mRNAs with complementary sequence and subsequently
leads to translation repression or mRNA degradation. Online sequence alignment
showed that there is a putative binding site of miR-223-3p on the 3'UTR of LIF,
which is considered to be an important marker of endometrial receptivity. Thus,
we hypothesized that miR-223-3p may affect embryo implantation by suppressing LIF
expression. In this study, we found that miR-223-3p and LIF protein was inversely
expressed in the endometrium of mice during implantation window. Then we proved
that miR-223-3p directly binds to LIF 3'UTR with luciferase reporter assay and
supresses the expression of LIF. To investigate whether miR-233-3p affects embryo
implantation, miR-223-3p agonist was injected into the uteri of pregnant mice.
The results demonstrated the suppressing effect of miR-223-3p on embryo
implantation. Furthermore, over-expression of miR-223-3p was found to compromise
pinopodes formation in the endometrium of mice. Taken all together, our findings
revealed that miR-223-3p suppresses pinopodes formation and LIF protein
expression, which may lead to diminished embryo implantation.