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Detection and Identification of Hematologic Malignancies and Solid Tumors by an
Electrochemical Technique
#MMPMID27115355
Wang Y
; Zhang B
; Zhang X
; Wang X
; Cheng J
; Chen B
PLoS One
2016[]; 11
(4
): e0153821
PMID27115355
show ga
PURPOSE: Develop and evaluate an electrochemical method to identify healthy
individuals, malignant hematopathic patients and solid tumor patients by
detecting the leukocytes in whole-blood. METHODS: A total of 114 individual blood
samples obtained from our affiliated hospital in China (June 2015- August 2015)
were divided into three groups: healthy individuals (n = 35), hematologic
malignancies (n = 41) and solid tumors (n = 38). An electrochemical workstation
system was used to measure differential pulse voltammetry due to the different
electrochemical behaviors of leukocytes in blood samples. Then, one-way analysis
of variance (ANOVA) was applied to analyze the scanning curves and to compare the
peak potential and peak current. RESULTS: The scanning curve demonstrated the
specific electrochemical behaviors of the blank potassium ferricyanide solution
and that mixed with blood samples in different groups. Significant differences in
mean peak potentials of mixture and shifts (?Ep (mV)) were observed of the three
groups (P< = 0.001). 106.00±9.00 and 3.14±7.48 for Group healthy individuals,
120.90±11.18 and 18.10±8.81 for Group hematologic malignancies, 136.84±11.53 and
32.89±10.50 for Group solid tumors, respectively. In contrast, there were no
significant differences in the peak currents and shifts. CONCLUSIONS: The newly
developed method to apply the electrochemical workstation system to identify
hematologic malignancies and solid tumors with good sensitivity and specificity
might be effective, suggesting a potential utility in clinical application.
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