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10.1038/srep25013 http://scihub22266oqcxt.onion/10.1038/srep25013 C4845006!4845006 !27113095     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27113095 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2016 ; 6 (ä): 25013 Nephropedia Template TP {{tp|p=27113095 |t=2016. p58(IPK) suppresses NLRP3 inflammasome activation and IL-1? production via inhibition of PKR in macrophages |pdf=|usr=}}{{27113095 }} gab.com Text p58(IPK) suppresses NLRP3 inflammasome activation and IL-1 production via inhibition of PKR in macrophages JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27113095 #FOAvip The NLRP3 inflammasome activation is a key signaling event for activation and secretion of pro-inflammatory cytokines such as IL-1? from macrophages. p58(IPK) is a molecular chaperone that regulates protein homeostasis through inhibiting eIF-2? kinases including double-stranded RNA-dependent protein kinase (PKR), which has been recently implicated in inflammasome activation. Herein we investigate the role of p58(IPK) in TLR4 signaling and inflammasome activation in macrophages. Primary bone marrow-derived macrophages (BMDM) was isolated from p58(IPK) knockout (KO) and wildtype (WT) mice and treated with lipopolysaccharide (LPS) and ATP to activate TLR4 signaling and stimulate inflammasome activation. Compared to WT macrophages, p58(IPK) deficient cells demonstrated significantly stronger activation of PKR, NF-?B, and JNK and higher expression of pro-inflammatory genes TNF-? and IL-1?. Coincidently, p58(IPK) deletion intensified NLRP3-inflammasome activation indicated by enhanced caspase 1 cleavage and increased IL-1? maturation and secretion. Pretreatment with specific PKR inhibitor or overexpression of p58(IPK) largely abolished the changes in inflammasome activation and IL-1? secretion in p58(IPK) null macrophages. Furthermore, immunoprecipitation assay confirmed the binding of p58(IPK) with PKR, but not other TLR4 downstream signaling molecules. Collectively, these results suggest a novel and crucial role of p58(IPK) in regulation of inflammasome activation and IL-1? secretion in macrophages. Twit Text FOAVip p58(IPK) suppresses NLRP3 inflammasome activation and IL-1 production via inhibition of PKR in macrophages ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27113095 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27113095 #FOAvip English Wikipedia <ref>{{Cite pmid|27113095 }}</ref> p58(IPK) suppresses NLRP3 inflammasome activation and IL-1? production via inhibition of PKR in macrophages #MMPMID27113095 Boriushkin E ; Wang JJ ; Li J ; Bhatta M ; Zhang SX Sci Rep 2016[Apr]; 6 (ä): 25013 PMID27113095 show ga The NLRP3 inflammasome activation is a key signaling event for activation and secretion of pro-inflammatory cytokines such as IL-1? from macrophages. p58(IPK) is a molecular chaperone that regulates protein homeostasis through inhibiting eIF-2? kinases including double-stranded RNA-dependent protein kinase (PKR), which has been recently implicated in inflammasome activation. Herein we investigate the role of p58(IPK) in TLR4 signaling and inflammasome activation in macrophages. Primary bone marrow-derived macrophages (BMDM) was isolated from p58(IPK) knockout (KO) and wildtype (WT) mice and treated with lipopolysaccharide (LPS) and ATP to activate TLR4 signaling and stimulate inflammasome activation. Compared to WT macrophages, p58(IPK) deficient cells demonstrated significantly stronger activation of PKR, NF-?B, and JNK and higher expression of pro-inflammatory genes TNF-? and IL-1?. Coincidently, p58(IPK) deletion intensified NLRP3-inflammasome activation indicated by enhanced caspase 1 cleavage and increased IL-1? maturation and secretion. Pretreatment with specific PKR inhibitor or overexpression of p58(IPK) largely abolished the changes in inflammasome activation and IL-1? secretion in p58(IPK) null macrophages. Furthermore, immunoprecipitation assay confirmed the binding of p58(IPK) with PKR, but not other TLR4 downstream signaling molecules. Collectively, these results suggest a novel and crucial role of p58(IPK) in regulation of inflammasome activation and IL-1? secretion in macrophages. |Adenosine Triphosphate/pharmacology [MESH] |Animals [MESH] |Cells, Cultured [MESH] |Gene Knockout Techniques [MESH] |HSP40 Heat-Shock Proteins/*genetics/metabolism [MESH] |Interleukin-1beta/*metabolism [MESH] |Lipopolysaccharides/pharmacology [MESH] |Macrophages/cytology/*metabolism [MESH] |Mice [MESH] |NLR Family, Pyrin Domain-Containing 3 Protein/*metabolism [MESH] |Protein Binding [MESH] |Signal Transduction [MESH]p58(IPK) suppresses NLRP3 inflammasome activation and IL-1? production(epmc).pdf DeepDyve Pubget Overpricing