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10.1128/JCM.00113-16 http://scihub22266oqcxt.onion/10.1128/JCM.00113-16 C4844727!4844727!26962084     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Microbiol 2016 ; 54 (5): 1335-9 Nephropedia Template TP {{tp|p=26962084|t=2016. Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus |pdf=|usr=}}{{26962084}} gab.com Text Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus JO: J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26962084 #FOAvip Rodent-borne hantaviruses cause two severe acute diseases: hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus pulmonary syndrome (HPS; also called hantavirus cardiopulmonary syndrome [HCPS]) in the Americas. Puumala virus (PUUV) is the most common causative agent of HFRS in Europe. Current routine diagnostic methods are based on serological analyses and can yield inconclusive results. Hantavirus-infected patients are viremic during the early phase of disease; therefore, detection of viral RNA genomes can be a valuable complement to existing serological methods. However, the high genomic sequence diversity of PUUV has hampered the development of molecular diagnostics, and currently no real-time reverse transcription-quantitative (RT)-PCR assay is available for routine diagnosis of HFRS. Here, we present a novel PUUV RT-PCR assay. The assay was validated for routine diagnosis of HFRS on samples collected in Sweden during the winter season from 2013 to 2014. The assay allowed detection of PUUV RNA in 98.7% of confirmed clinical HFRS samples collected within 8 days after symptomatic onset. In summary, this study shows that real-time RT-PCR can be a reliable alternative to serological tests during the early phase of HFRS. Twit Text FOAVip Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus ????J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26962084 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26962084 #FOAvip English Wikipedia <ref>{{Cite pmid|26962084}}</ref> Molecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by Puumala Virus #MMPMID26962084 Lagerqvist N; Hagström Å; Lundahl M; Nilsson E; Juremalm M; Larsson I; Alm E; Bucht G; Ahlm C; Klingström J J Clin Microbiol 2016[May]; 54 (5): 1335-9 PMID26962084show ga Rodent-borne hantaviruses cause two severe acute diseases: hemorrhagic fever with renal syndrome (HFRS) in Eurasia, and hantavirus pulmonary syndrome (HPS; also called hantavirus cardiopulmonary syndrome [HCPS]) in the Americas. Puumala virus (PUUV) is the most common causative agent of HFRS in Europe. Current routine diagnostic methods are based on serological analyses and can yield inconclusive results. Hantavirus-infected patients are viremic during the early phase of disease; therefore, detection of viral RNA genomes can be a valuable complement to existing serological methods. However, the high genomic sequence diversity of PUUV has hampered the development of molecular diagnostics, and currently no real-time reverse transcription-quantitative (RT)-PCR assay is available for routine diagnosis of HFRS. Here, we present a novel PUUV RT-PCR assay. The assay was validated for routine diagnosis of HFRS on samples collected in Sweden during the winter season from 2013 to 2014. The assay allowed detection of PUUV RNA in 98.7% of confirmed clinical HFRS samples collected within 8 days after symptomatic onset. In summary, this study shows that real-time RT-PCR can be a reliable alternative to serological tests during the early phase of HFRS. äMolecular Diagnosis of Hemorrhagic Fever with Renal Syndrome Caused by(epmc).pdf DeepDyve Pubget Overpricing