Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.molcel.2016.03.007 http://scihub22266oqcxt.onion/10.1016/j.molcel.2016.03.007 C4842148!4842148!27067601     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2016 ; 62 (2): 237-47 Nephropedia Template TP {{tp|p=27067601|t=2016. Enhancer regulation of transcriptional bursting parameters revealed by forced chromatin looping |pdf=|usr=}}{{27067601}} gab.com Text Enhancer regulation of transcriptional bursting parameters revealed by forced chromatin looping JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27067601 #FOAvip Mammalian genes do not transcribe RNA continuously but in bursts. Transcriptional output can be modulated by altering burst fraction or burst size, but how regulatory elements control bursting parameters remains unclear. Single-molecule RNA FISH experiments revealed that the ?-globin enhancer (LCR) predominantly augments transcriptional burst fraction of the ?-globin gene with modest stimulation of burst size. To specifically measure the impact of long range chromatin contacts on transcriptional bursting, we forced an LCR-?-globin promoter chromatin loop. We observed that raising contact frequencies increases burst fraction but not burst size. In cells in which two developmentally distinct LCR-regulated globin genes are cotranscribed in cis, burst sizes of both genes are comparable. However, allelic co-transcription of both genes is statistically disfavored, suggesting mutually exclusive LCR-gene contacts. These results are consistent with competition between the ?-type globin genes for LCR contacts and suggest that LCR-promoter loops are formed and released with rapid kinetics. Twit Text FOAVip Enhancer regulation of transcriptional bursting parameters revealed by forced chromatin looping ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27067601 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27067601 #FOAvip English Wikipedia <ref>{{Cite pmid|27067601}}</ref> Enhancer regulation of transcriptional bursting parameters revealed by forced chromatin looping #MMPMID27067601 Bartman CR; Hsu SC; Hsiung CCS; Raj A; Blobel GA Mol Cell 2016[Apr]; 62 (2): 237-47 PMID27067601show ga Mammalian genes do not transcribe RNA continuously but in bursts. Transcriptional output can be modulated by altering burst fraction or burst size, but how regulatory elements control bursting parameters remains unclear. Single-molecule RNA FISH experiments revealed that the ?-globin enhancer (LCR) predominantly augments transcriptional burst fraction of the ?-globin gene with modest stimulation of burst size. To specifically measure the impact of long range chromatin contacts on transcriptional bursting, we forced an LCR-?-globin promoter chromatin loop. We observed that raising contact frequencies increases burst fraction but not burst size. In cells in which two developmentally distinct LCR-regulated globin genes are cotranscribed in cis, burst sizes of both genes are comparable. However, allelic co-transcription of both genes is statistically disfavored, suggesting mutually exclusive LCR-gene contacts. These results are consistent with competition between the ?-type globin genes for LCR contacts and suggest that LCR-promoter loops are formed and released with rapid kinetics. äEnhancer regulation of transcriptional bursting parameters revealed by(epmc).pdf DeepDyve Pubget Overpricing