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Hydrogen-Rich Saline Promotes the Recovery of Renal Function after
Ischemia/Reperfusion Injury in Rats via Anti-apoptosis and Anti-inflammation
#MMPMID27148060
Li J
; Hong Z
; Liu H
; Zhou J
; Cui L
; Yuan S
; Chu X
; Yu P
Front Pharmacol
2016[]; 7
(?): 106
PMID27148060
show ga
PURPOSE: Hydrogen is a proven novel antioxidant that selectively reduces hydroxyl
radicals. In this study, we investigated the effects of hydrogen-rich saline
solution on the prevention of renal injury induced by ischemia/reperfusion (I/R)
and on renal function recovery. METHODS: A rat model of renal I/R injury was
induced by 45 min occlusion of the left renal pedicle, followed by 108 h
reperfusion. The right kidney was surgically removed. Then, 0.9% NaCl solution (1
ml/kg) or hydrogen-rich saline solution (HRSS; 1 ml/kg) was injected into the
abdominal cavity at 4 h intervals. We assessed the influence of HRSS or control
saline solution on the recovery of renal function after I/R injury. Kidney
tissues were taken at different time points (24, 36, 48, 72, and 108 h after
reperfusion) and frozen (-80°C). Kidney cell apoptosis was evaluated using
terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive
staining. Additionally, the apoptotic factors (Bcl-2, Bax, caspase-3, caspase-9,
and caspase-8) and the pro-inflammatory cytokines (IL-6 and TNF-?) were measured
in the kidney tissues. Finally, serum blood urea nitrogen (BUN) and creatinine
(Cr) levels were measured. RESULTS: Histological analyses revealed a marked
reduction of interstitial congestion, edema and hemorrhage in renal tissue after
HRSS treatment compared to saline treatment. After I/R injury, BUN, Cr, Bcl-2,
caspase-3, caspase-9, caspase-8, IL-6, and TNF-? were all significantly
increased, while Bax expression was decreased. HRSS remarkably reversed these
changes. Moreover, BUN and Cr decreased more rapidly in the rats treated with
HRSS compared to the rats treated with control saline solution. CONCLUSIONS: HRSS
showed a protective effect in the prevention of renal injury and could promote
renal function recovery after I/R injury in rats. HRSS might partially exert its
role through an anti-apoptotic and anti-inflammatory action in kidney cells.
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