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10.1371/journal.pone.0153252 http://scihub22266oqcxt.onion/10.1371/journal.pone.0153252 C4839700!4839700 !27100186     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27100186 &cmd=llinks): Failed to open stream: HTTP request failed! 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IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood |pdf=|usr=}}{{27100186 }} gab.com Text IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27100186 #FOAvip Diagnosis of chronic glomerulonephritis (CGN) depends primarily on renal biopsy, which is expensive and requires hospitalization, creating a demand for noninvasive diagnostic method for this disease. We used DNA microarray analysis to search for genes whose expression levels in peripheral blood mononuclear cells (PBMCs) could distinguish between patients with CGN and healthy volunteers (HVs). We selected immunoglobulin A nephropathy (IgAN) and membranous nephropathy (MN) as typical forms of CGN. The mRNA level of the gene encoding interferon (IFN)-alpha-inducible protein 27, IFI27, which is preferentially expressed in podocytes of glomeruli, was lower in PBMCs of IgAN and MN patients than in those of HVs. This result was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Moreover, qRT-PCR analysis revealed that the IFI27 mRNA level was reduced in PBMCs of patients with other types of chronic glomerulonephritis. IFI27 immunohistochemical staining of biopsied specimens also confirmed reduced expression of IFI27 protein in IgAN and MN patients. Based on these results, we propose that IFI27 could serve as a noninvasive diagnostic marker for CGNs using peripheral blood. Twit Text FOAVip IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27100186 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27100186 #FOAvip English Wikipedia <ref>{{Cite pmid|27100186 }}</ref> IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood #MMPMID27100186 Nagasawa Y ; Okuzaki D ; Muso E ; Yamamoto R ; Shinzawa M ; Iwasaki Y ; Iwatani H ; Nakanishi T ; Isaka Y ; Nojima H PLoS One 2016[]; 11 (4 ): e0153252 PMID27100186 show ga Diagnosis of chronic glomerulonephritis (CGN) depends primarily on renal biopsy, which is expensive and requires hospitalization, creating a demand for noninvasive diagnostic method for this disease. We used DNA microarray analysis to search for genes whose expression levels in peripheral blood mononuclear cells (PBMCs) could distinguish between patients with CGN and healthy volunteers (HVs). We selected immunoglobulin A nephropathy (IgAN) and membranous nephropathy (MN) as typical forms of CGN. The mRNA level of the gene encoding interferon (IFN)-alpha-inducible protein 27, IFI27, which is preferentially expressed in podocytes of glomeruli, was lower in PBMCs of IgAN and MN patients than in those of HVs. This result was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Moreover, qRT-PCR analysis revealed that the IFI27 mRNA level was reduced in PBMCs of patients with other types of chronic glomerulonephritis. IFI27 immunohistochemical staining of biopsied specimens also confirmed reduced expression of IFI27 protein in IgAN and MN patients. Based on these results, we propose that IFI27 could serve as a noninvasive diagnostic marker for CGNs using peripheral blood. |Gene Expression Regulation [MESH] |Genetic Markers [MESH] |Glomerulonephritis, IGA/blood/diagnosis/*genetics [MESH] |Glomerulonephritis, Membranous/blood/diagnosis/*genetics [MESH] |Humans [MESH] |Immunohistochemistry [MESH] |Leukocytes, Mononuclear/metabolism [MESH]IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nep(epmc).pdf DeepDyve Pubget Overpricing