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10.1038/srep24765 http://scihub22266oqcxt.onion/10.1038/srep24765 C4838866!4838866 !27097775     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27097775 &cmd=llinks): Failed to open stream: HTTP request failed! 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CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations |pdf=|usr=}}{{27097775 }} gab.com Text CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27097775 #FOAvip The CRISPR/Cas9 system has successfully been used in various organisms for precise targeted gene editing. Although it has been demonstrated that CRISPR/Cas9 system can induce mutation in tomato plants, the stability of heredity in later generations and mutant specificity induced by the CRISPR/Cas9 system in tomato plants have not yet been elucidated in detail. In this study, two genes, SlPDS and SlPIF4, were used for testing targeted mutagenesis in tomato plants through an Agrobacterium tumefaciens-mediated transformation method. A high mutation frequency was observed in all tested targets in the T0 transgenic tomato plants, with an average frequency of 83.56%. Clear albino phenotypes were observed for the psd mutants. High frequencies of homozygous and biallelic mutants were detected even in T0 plants. The majority of the detected mutations were 1- to 3-nucleotide deletions, followed by 1-bp insertions. The target mutations in the T0 lines were stably transmitted to the T1 and T2 generations, without new modifications or revision. Off-target activities associated with SlPDS and SlPIF4 were also evaluated by sequencing the putative off-target sites, and no clear off-target events were detected. Our results demonstrate that the CRISPR/Cas9 system is an efficient tool for generating stable and heritable modifications in tomato plants. Twit Text FOAVip CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27097775 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27097775 #FOAvip English Wikipedia <ref>{{Cite pmid|27097775 }}</ref> CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations #MMPMID27097775 Pan C ; Ye L ; Qin L ; Liu X ; He Y ; Wang J ; Chen L ; Lu G Sci Rep 2016[Apr]; 6 (ä): 24765 PMID27097775 show ga The CRISPR/Cas9 system has successfully been used in various organisms for precise targeted gene editing. Although it has been demonstrated that CRISPR/Cas9 system can induce mutation in tomato plants, the stability of heredity in later generations and mutant specificity induced by the CRISPR/Cas9 system in tomato plants have not yet been elucidated in detail. In this study, two genes, SlPDS and SlPIF4, were used for testing targeted mutagenesis in tomato plants through an Agrobacterium tumefaciens-mediated transformation method. A high mutation frequency was observed in all tested targets in the T0 transgenic tomato plants, with an average frequency of 83.56%. Clear albino phenotypes were observed for the psd mutants. High frequencies of homozygous and biallelic mutants were detected even in T0 plants. The majority of the detected mutations were 1- to 3-nucleotide deletions, followed by 1-bp insertions. The target mutations in the T0 lines were stably transmitted to the T1 and T2 generations, without new modifications or revision. Off-target activities associated with SlPDS and SlPIF4 were also evaluated by sequencing the putative off-target sites, and no clear off-target events were detected. Our results demonstrate that the CRISPR/Cas9 system is an efficient tool for generating stable and heritable modifications in tomato plants. |*CRISPR-Cas Systems [MESH] |*Gene Targeting [MESH] |*Mutagenesis [MESH] |Genes, Plant [MESH] |Genome, Plant [MESH] |Genotype [MESH] |Inheritance Patterns [MESH] |Mutation [MESH] |Phenotype [MESH] |Plants, Genetically Modified [MESH] |RNA, Guide, CRISPR-Cas Systems/genetics [MESH]CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in t(epmc).pdf DeepDyve Pubget Overpricing