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10.1002/pro.2914 http://scihub22266oqcxt.onion/10.1002/pro.2914 C4838651!4838651!26941080     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Protein+Sci 2016 ; 25 (5): 1061-8 Nephropedia Template TP {{tp|p=26941080|t=2016. Dodine as a transparent protein denaturant for circular dichroism and infrared studies |pdf=|usr=}}{{26941080}} gab.com Text Dodine as a transparent protein denaturant for circular dichroism and infrared studies JO: Protein Sci http://www.kidney.de/pget.php?&tw=1&pmid=26941080 #FOAvip The fungicide dodine combines the cooperative denaturation properties of guanidine with the mM denaturation activity of SDS. It was previously tested only on two small model proteins. Here we show that it can be used as a chemical denaturant for phosphoglycerate kinase (PGK), a much larger two?domain enzyme. In addition to its properties as a chemical denaturant, dodine facilitates thermal denaturation of PGK, and we show for the first time that it also facilitates pressure denaturation of a protein. Much higher quality circular dichroism and amide I? infrared spectra of PGK can be obtained in dodine than in guanidine, opening the possibility for use of dodine as a denaturant when UV or IR detection is desirable. One caution is that dodine denaturation, like other detergent?based denaturants, is less reversible than guanidine denaturation. Twit Text FOAVip Dodine as a transparent protein denaturant for circular dichroism and infrared studies ????Protein Sci http://www.kidney.de/pget.php?&tw=1&pmid=26941080 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26941080 #FOAvip English Wikipedia <ref>{{Cite pmid|26941080}}</ref> Dodine as a transparent protein denaturant for circular dichroism and infrared studies #MMPMID26941080 Guin D; Sye K; Dave K; Gruebele M Protein Sci 2016[May]; 25 (5): 1061-8 PMID26941080show ga The fungicide dodine combines the cooperative denaturation properties of guanidine with the mM denaturation activity of SDS. It was previously tested only on two small model proteins. Here we show that it can be used as a chemical denaturant for phosphoglycerate kinase (PGK), a much larger two?domain enzyme. In addition to its properties as a chemical denaturant, dodine facilitates thermal denaturation of PGK, and we show for the first time that it also facilitates pressure denaturation of a protein. Much higher quality circular dichroism and amide I? infrared spectra of PGK can be obtained in dodine than in guanidine, opening the possibility for use of dodine as a denaturant when UV or IR detection is desirable. One caution is that dodine denaturation, like other detergent?based denaturants, is less reversible than guanidine denaturation. äDodine as a transparent protein denaturant for circular dichroism and (epmc).pdf DeepDyve Pubget Overpricing