Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1371/journal.pone.0153360 http://scihub22266oqcxt.onion/10.1371/journal.pone.0153360 C4838334!4838334 !27096746     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27096746 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2016 ; 11 (4 ): e0153360 Nephropedia Template TP {{tp|p=27096746 |t=2016. Design of Peptide Substrate for Sensitively and Specifically Detecting Two A?-Degrading Enzymes: Neprilysin and Angiotensin-Converting Enzyme |pdf=|usr=}}{{27096746 }} gab.com Text Design of Peptide Substrate for Sensitively and Specifically Detecting Two A -Degrading Enzymes: Neprilysin and Angiotensin-Converting Enzyme JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27096746 #FOAvip Upregulation of neprilysin (NEP) to reduce A? accumulation in the brain is a promising strategy for the prevention of Alzheimer's disease (AD). This report describes the design and synthesis of a quenched fluorogenic peptide substrate qf-A?(12-16)AAC (with the sequence VHHQKAAC), which has a fluorophore, Alexa-350, linked to the side-chain of its C-terminal cysteine and a quencher, Dabcyl, linked to its N-terminus. This peptide emitted strong fluorescence upon cleavage. Our results showed that qf-A?(12-16)AAC is more sensitive to NEP than the previously reported peptide substrates, so that concentrations of NEP as low as 0.03 nM could be detected at peptide concentration of 2 ?M. Moreover, qf-A?(12-16)AAC had superior enzymatic specificity for both NEP and angiotensin-converting enzyme (ACE), but was inert with other A?-degrading enzymes. This peptide, used in conjunction with a previously reported peptide substrate qf-A?(1-7)C [which is sensitive to NEP and insulin-degrading enzyme (IDE)], could be used for high-throughput screening of compounds that only upregulate NEP. The experimental results of cell-based activity assays using both qf-A?(1-7)C and qf-A?(12-16)AAC as the substrates confirm that somatostatin treatment most likely upregulates IDE, but not NEP, in neuroblastoma cells. Twit Text FOAVip Design of Peptide Substrate for Sensitively and Specifically Detecting Two A -Degrading Enzymes: Neprilysin and Angiotensin-Converting Enzyme ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=27096746 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27096746 #FOAvip English Wikipedia <ref>{{Cite pmid|27096746 }}</ref> Design of Peptide Substrate for Sensitively and Specifically Detecting Two A?-Degrading Enzymes: Neprilysin and Angiotensin-Converting Enzyme #MMPMID27096746 Chen PT ; Chen CL ; Lin LT ; Lo CH ; Hu CJ ; Chen RP ; Wang SS PLoS One 2016[]; 11 (4 ): e0153360 PMID27096746 show ga Upregulation of neprilysin (NEP) to reduce A? accumulation in the brain is a promising strategy for the prevention of Alzheimer's disease (AD). This report describes the design and synthesis of a quenched fluorogenic peptide substrate qf-A?(12-16)AAC (with the sequence VHHQKAAC), which has a fluorophore, Alexa-350, linked to the side-chain of its C-terminal cysteine and a quencher, Dabcyl, linked to its N-terminus. This peptide emitted strong fluorescence upon cleavage. Our results showed that qf-A?(12-16)AAC is more sensitive to NEP than the previously reported peptide substrates, so that concentrations of NEP as low as 0.03 nM could be detected at peptide concentration of 2 ?M. Moreover, qf-A?(12-16)AAC had superior enzymatic specificity for both NEP and angiotensin-converting enzyme (ACE), but was inert with other A?-degrading enzymes. This peptide, used in conjunction with a previously reported peptide substrate qf-A?(1-7)C [which is sensitive to NEP and insulin-degrading enzyme (IDE)], could be used for high-throughput screening of compounds that only upregulate NEP. The experimental results of cell-based activity assays using both qf-A?(1-7)C and qf-A?(12-16)AAC as the substrates confirm that somatostatin treatment most likely upregulates IDE, but not NEP, in neuroblastoma cells. |*Proteolysis [MESH] |Acetates/chemistry/metabolism [MESH] |Alzheimer Disease/*enzymology/metabolism [MESH] |Amino Acid Sequence [MESH] |Amyloid beta-Peptides/chemistry/*metabolism [MESH] |Cell Line, Tumor [MESH] |Chromones/chemistry/metabolism [MESH] |Humans [MESH] |Insulysin/metabolism [MESH] |Neprilysin/*metabolism [MESH] |Peptide Fragments/chemistry/*metabolism [MESH] |Peptidyl-Dipeptidase A/*metabolism [MESH] |Spectrometry, Fluorescence/methods [MESH]Design of Peptide Substrate for Sensitively and Specifically Detecting(epmc).pdf DeepDyve Pubget Overpricing