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2016 ; 89
(5
): 1153-1159
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English Wikipedia
A label-free approach by infrared spectroscopic imaging for interrogating the
biochemistry of diabetic nephropathy progression
#MMPMID26924056
Varma VK
; Kajdacsy-Balla A
; Akkina SK
; Setty S
; Walsh MJ
Kidney Int
2016[May]; 89
(5
): 1153-1159
PMID26924056
show ga
Routine histology, the current gold standard, involves staining for specific
biomolecules. However, untapped biochemical information in tissue can be gathered
using biochemical imaging. Infrared spectroscopy is an emerging modality that
allows label-free chemical imaging to derive biochemical information (such as
protein, lipids, DNA, collagen) from tissues. Here we employed this technology in
order to better predict the development of diabetic nephropathy. Using human
primary kidney biopsies or nephrectomies, we obtained tissue from 4
histologically normal kidneys, 4 histologically normal kidneys from diabetic
subjects, and 5 kidneys with evidence of diabetic nephropathy. A biochemical
signature of diabetic nephropathy was derived that enabled prediction of
nephropathy based on the ratio of only 2 spectral frequencies. Nonetheless, using
the entire spectrum of biochemical information, we were able to detect renal
disease with near-perfect accuracy. Additionally, study of sequential protocol
biopsies from 3 transplanted kidneys showed biochemical changes even prior to
clinical manifestation of diabetic nephropathy. Thus, infrared imaging can
identify critical biochemical alterations that precede morphologic changes,
potentially allowing for earlier intervention.
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